XB-CLONE-4350574
Clone Name: IMAGE:7025900
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Gene:
mospd2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081354
[+]
GATAACTAGATGAGAGAAGCGGTGTGTGGCGATGGCTGAGGACAAGGAACAACTAATTCAGGAGACAAGAAAAAGGTTTACTCAGGAGTATCTGCAAGAT
AAGGCTGACAGATATGATTCTCGTGATGTGGAAAGACTACAGAAGGATGACACCTTGGTGGAAAGCTACCTTATGTGGAGGCATTATGTAACAGAGGAAG
CTCTAAAGATGATGGATGAAAGCCTGAAATGGAGGAAAGACATTGGTGTAAATGACTTGAATGAATCCACTATCCCTAAGTGGTGTTTTGAGACTGGAGC
ATCTTATCTGCATGGATATGATAAAGAAGGCAATAAATTGTTGTGGCTCAAGGTAAAGCTCCATGTACGTGATGGTAAAACAACTGAGGACAAGAAAAAG
TTTGTTGCATTCTGGTTGGAGCGCTATGCCAGGAGAGAACCAGGGAAGTTCATAACTGTTGTTTTTGACATGGTTGACTCAGGACTAAGCAATGTAGATA
TGGATTTTGTTCGGTTTGTCGTCAACTCTTTTAAGACCTACTACCCCAGATATCTGTCTAAGATGGTAATTTATGAAATGCCGTGGATATTGAATGCTGC
CTTCAAAATTGTGAAAAGTTGGCTTGGTCCAGAGGCTATAAGTTTGCTGAAATTTGCAAACAAGAATCAAGTTCAGGACTACATCAGTGCAGAATACTTG
CCACCTCATATGGGAGGAACAGATCCCTTCAAGTATAGTTATCCTCCATTGCCTGATGATGACTTTCAAACGCCACTGTGTGAGAATGGACCAGTAGCAA
GTGAGGATGATTCCGAAAGTAAACTGAGTGAATCAGATCTCCGTGACACCGTAGATTTAAGTGTGCCAAAAAAGATAAGCATCAGCGACGACCAAATTAA
ACAGGAGGATGTGGAAAAAAATGATGTAAAGCAAAAAAACAACAGGAAGGCATCAACTGTATTCAAAGGACCTTTGTTACATATCAGTCCAGCAGAAGAA
CTGCATTTTGGGTCCAAGGAAAGTGGAGAGCGAAAATGTGTAATTGTGCTGAATAATGTAACAAAAAACACAGTAGCATTTAAGGTGAGAACAACAGCTC
CTGAAAAATACAGAGTCAAACCAAGTAACAGCAGCTGCGAACCAGGATCCACAGTTGATATTGTGGTGTCTCTGCATGCAGCCTTTGTAGCATCTCTCCA
GGACAGATTCTTAGTAATGGCGGCAGAAATGGAGCAGCCCTCCGGTGCCAGCACACAAGAACTCACACAGTTTTGGAAGGAGGTGCCCAGGAGTAAAGTC
ATGGAGCATAGGTTAAGATGCCACGTTGTTGAAACTAGCAAGCCTGCCTCATTAATCCTAAAATCAAACACAATGAACCACTCTGTATCGGACAGTGACG
ACTTGCATTTTAAGCTCAATCAGCTGTTGGAAACTAACAGAAGGTTAGAAGCACAGATGGGGCACTGCCTCTGGTTTCAGCACATGTTAGCCACCTTATT
ATCCATTCTGGTCGCCATTGTTGCTGCTGGAGTGTATATAACGAAACACCAGGACTGTTAAATGTGTTGTTCATCTCCCAGATGCGCTGCCTATGAAACC
ACCTGTGAGAGAAATGGATCTGCCCCCACATGACCAAAGATGCCTTCAAAGCAGTGTTATGTTGTATAAGACAGCTAATTCATAAAGAGGTTACGACGTT
CCCCAGACGTTTAAATGATGCCAATATTTGGTGTAACTTTGCTTATTGTCTTTAGTGGTAAAGCTGTGAAACAGTAATGTAATGAAGTACAGCTCGTAGA
CAGGCCAGCCGCCATGACCCTGAATGTTATATTAATGTGAGCCTTGGATACTTCTCGTGATAATATTTAAGTCCTAAGCAGATGTTGGCAAATGGTATGC
ACTACAAAACTCTTATGGATCCTGTGAATTTACCATATAGACTGCTGCTCATCTTGCTTATCTTTCTTCTAGTTTGCAGTTAAATGTTTAGTTGCCATAG
TTCTTACTTGCCATAAATACCGGAGCGTTTAGCTGTCCCCAGCAAAGGTATTTTTTTATCACTAATAAATTAGTGGCGAACATTTGCTTTAATTTATGTG
CAATCCTCAGCGGCTTACATCCCATGAGTCAGTCGGTGCATATAGATGCTGCGATACTGTGCTAACTACTAAAACCGTAGAATTCAGTCACGCCATGGGT
ATTTATTTGCTCTTGTCATAGATGAAACCCAGGAACCCCCAGAGGCCATTTGTTAAGAAGGACAGTATTGACAGAACATTGCTTTTCTGTTACATGGATG
AAGGATAAATGCATATAGTGCATCAAGCCAATTATAACCTCAGGTTTCAGTCCCTCCTTCCCTCGCTGCTACTGATAATGTAAATAAATGTTTTTATGAT
ACTATTTGGTGTCATAGTCTCACTGCTTATGCCTTCTTGGATGACGTCCTCTATTTTTGTATATAAATGAACATTTGGGCACCCGTAAAAAAAAAAAAAA
AAAAAAAAA
5' EST: CF785439
[+]taattaatcccccccccccccgataactagatgagagaagcggtgtgtggcgatggctgaggacaaggaacaactaattcaggagacaagaaaaaggttt
actcaggagtatctgcaagataaggctgacagatatgattctcgtgatgtggaaagactacagaaggatgacaccttggtggaaagctaccttatgtgga
ggcattatgtaacagaggaagctctaaagatgatggatganagcctgaaatggaggaaagacattggtgtaaatgacttgaatgaatccactatccctaa
gtggtgttttgagactggagcatcttatctgcatggatatgataaagaaggcaataaattgttgtggctcaaggtaaagctccatgtacgtgatggtaaa
acaactgangacaagaaaaagtttgttgcattctggttggagcgctatgccaggagagaaccagggaagttcataactgttgtttttgacatggttgact
caggactaagcaatgtagatatggattttgttcggtttgtcgtncactcttttaagacctactaccccagatatctgtctaagatggtaatttatgaaat
gccgtggatattgaatgctgccttcaaattngtgaaaagttggcttggtccagaagctataagtttgctgaaatttgcaaacagaatcagttcaggacta
catcagtgcagaatacttgccacctcatatgggaggaacagatcccttcaagtataggttatctccattgcctgatgatgactttcaaacgccactgtgt
ga
3' EST: CF785440
[+]ttttttttttttttttttttttacgggtgcccaaatgttcatttatatacaaaaatagaggacgtcatccaagaaggcataagcagtgagactatgacac
caaatagtatcataaaaacatttatttacattatcagtagcagcgagggaaggagggactgaaacctgaggttataattggcttgatgcactatatgcat
ttatccttcatccatgtaacagaaaagcaatgttctgtcaatactgtccttcttaacaaatggcctctgggggttcctgggtttcatctatgacaagagc
aaataaatacccatggcgtgactgaattctacggttttagtagttagcacagtatcgcagcatctatatgcaccgactgactcatgggatgtaagccgct
gaggattgcacataaattaaagcaaatgttcgccactaatttattagtgataaaaaaatacctttgctggggacagctaaacgctccggtatttatggca
agtaagaactatggcaactaaacatttaactgcaaactagaagaaagataagcaagatgagcagcagtctatatggtaaattcacaggatccataagagt
tttgtagtgcataccatttgccaacatctgcttaggacttaaatattatcacgagaagtatccaaggctcacattaatataacattcagggtcatggcgg
ctggcctgtctacgagctgtacttcattacattactgtttcacagctttaccactaaagacaataagcaaagttacaccaaatattggcatcattttaac
gtctgggggaacgtcgtaacctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: