XB-CLONE-4350565
Clone Name: IMAGE:7025896
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Gene:
acp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077004 [+]
GGAAACTTACTTAGCAGGCGACATGGCGCAGGGCAGCGGCAAGTCCGTGTTATTCGTATGCTTGGGTAATATCTGTCGATCTCCAATAGCTGAAGCTGTT
TTTCGGAAGTTGGTTACCGATGCAGGAACATCTAAGGAGTGGTCCATAGACAGCGCTGCAACTTCAGACTGGAATGTGGGTTCTTCCCCAGATTCACGGG
CCCTAAAGTGCTTAAAAAGCCATGGTATCGAAACAGCTCATAGAGCACAGCAGATTACAAGAGATGATTTTCTTTCTTATGACTATATTCTGTGCATGGA
TGAAAGTAATCTACGAGATTTGAAGAGAAAAGGCAGCCAGGTGCAAAACAGCAAAGCTAAAATTGAACTGCTTGGTAGCTACGACCCACAAAAAAAGCTC
ATTATCGAGGATCCCTACTATGGTAGGGATGAAGACTTTGAGACCGTTTATCAGCAATGTATTAGATGCTGCAAATCATTTTTGGAAAGAACTTAACCAT
GAGCAGAAGAACAAACTGAAGATTAATCTGCTTTTCCCTGATTCTCTGGTGTTTCACCGTTACCATGTTTTACTGCTTTGCCGGGAAGCCAAGCCTGCAG
TTCTTTTGTGTTCCATACAGAATGTTGTACAACACCAGAGTGTGGCCCTCTGTTGGGCATCAAATGAATAGTACCTTCTTTGTGATTATATAAATAAAGA
AGTTTTAATATAGCAAAAAAAAAAAAAAAA
5' EST: CF785444 [+]taattaatcccccccccccccggaaacttacttagcaggcgacatggcgcagggcagcggcaagtccgtgttattcgtatgcttgggtaatatctgtcga
tctccaatagctgaagctgtttttcggaagttggttaccgatgcaggaacatctaaggagtggtccatagacagcgctgcaacttcagactggaatgtgg
gttcttccccagattcacgggccctaaagtgcttaaaaagccatggtatcgaaacagctcatagagcacagcagattacaagagatgattttctttctta
tgactatattctgtgcatggatgaaagtaatctacgagatttgaagagaaaaggcagccaggtgcaaaacagcaaagctaaaattgaactgcttggtagc
tacgacccacaaaaaaagctcattatcgaggatccctactatggtagggatgaagactttgagaccgtttatcagcaatgtattagatgctgcaaatcat
ttttggaaagaacttaaccatgagcagaagaacaaactgaagattaatctgcttttccctgattctctggtgtttcaccgttaccatgttttactgcttt
gccgggaagccaagcctgcagttcttttgtgttccatacagaatgttgtacaacaccagagtgtggccctctgttgggcatcaaatgaatagtacctttc
cttgggattatataaataaagaaagtttttatac
3' EST: CF785445 [+]tttttttttttttttgctatattaaaacttctttatttatataatcacaaagaaggtactattcatttgatgcccaacagagggccacactctggtgttg
tacaacattctgtatggaacacaaaagaactgcaggcttggcttcccggcaaagcagtaaaacatggtaacgtgaacaccagagatcaggaaaagagaaa
tatctcagtttgtctctgctcatgttaagtctttccaaatgattgcacctccaaaaaattggcgaaaaaacggcccaaatctttccccccacccaaaaag
gatccccaaaaagaccttttttggggggggggaacccaaacaggaaattttacctttggggtttgaacccgggccccttttttttcttaaaaccgaaaaa
aattttcccccccgcccaaaaaattttcttaaaaaaaaaaaaaaaccccccttaaaaaaagggggggggcccaaaaaaaagtttttttaaaaaaacgggg
gggttttttttaaaaacatattggcgcccccccctttttttgtggggaaaaaaaacaacaccccccccccccccccaaaaaaaaaaccccccccccccct
ttttttttgaacccccccccccttctattttttttttttctccccccccccgnggcggcgcaccccctcctttaaaaaaaaaaaaaaaaaccctccctct
ttttttattatataaaaaaaaaaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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