XB-CLONE-4350562
Clone Name: IMAGE:7025895
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Gene:
rpl12
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785447 [+]
taattaatcccccccccgcctctttctcgtcctgctttaccgctggtagaggtgcaactacttatcggtcgtcccgaatctgggttcatccgacaccgaa
accggcccgagtgaagcggactgcccctagtattctgccgccatgcctcccaaattcgaccccaccgagattaaagtcgtgttcctgagatgtactggtg
gggaggtgggagccacttctgctctggcccctaaaatcggacctttgggtttgtctcccaaaaaagtgggggatgacattgccaaggctaccggtgactg
gaaggggctgaggatcacagtcaaactgaccatccagaacagacaggcccagattgaggttgtgccatctgcctccgccctgatcattaaagccctgaag
gagcctccccgtgacaggaagaagcagaagaacattaaacacaacggcaacatctccatggacgaagtgatcagcatcgcccgccagatgaggcaccgat
ccctggccagggagctttccggaaccattaaggagatcctgggaaccgcacagtccgtanggtgcaacgtggatgggagacacccccatgacatcattga
cgacatcaacagcggcgcagtagagtgcccggcgagctaaacctgccctggggagcaatgggattcctcaataaagagagattaacagtcgaaaaaaaaa
aaaaaaaagatcccactagtttctagagcgggccgccaccggcggtgggagctttggccgtgggccccaacttggttttattgccagccttaataatggg
tttacaaaattaaaagcattagccattcacc
3' EST: CF785446 [+]tttttttttttttttcgactgttaatctctctttattgaggaatcccattggctcccagggcaggtttagctcgccgggcactctactgcgccgctgttg
atgtcgtcaatgatgtcatgggggtgtctcccatccacgttgcaccctacggactgtgcggttcccaggatctccttaatggttccggaaagctccctgg
ccagggatcggtgcctcatctggcgggcgatgctgatcacttcgtccatggagatgttgccgttgtgtttaatgttcttctgcttcttcctgtcacgggg
aggctccttcagggctttaatgatcagggcggaggcagatggcacaacctcaatctgggcctgtctgttctggatggtcagtttgactgtgatcctcagc
cccttccagtcaccggtagccttggcaatgtcatcccccacttttttgggagacaaacccaaaggtccgattttaggggccagagcagaagtggctccca
cctccccaccagtacatctcaggaacacgactttaatctcggtggggtcgaatttgggaggcatggcggcagaatactaggggcagtccgcttcactcgg
gccggtttcggtgtcggatgaacccagattcgggacgaccgataagtagttgcacctctaccagcggttaaagcaagacgaaaaaaaagccggggggggg
gattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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