XB-CLONE-4350559
Clone Name: IMAGE:7025894
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Gene:
myl6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785448
[+]
taattaatcccccccccccccatgcttccttaatgctgtagtgtgcagagaaagataaattatttcaaatttctattttttcccctatccaacatcaaat
aaactgagtgactttttccaagacatcttgtgatttgtttcatttctctgcacaagttacatcttanaatcattctgaggggtagatgcatcaggagaaa
gggaaagaattaattttacagggctcttgctgaacatacattctgcatactattttaatgatggtttctgttatacctatacaagacagcagcagtcatt
tctttgttctgctgagtgttatcaaagggagaaaatagtaaaatacattaatagtccacaaataaggtttgtaatcctttttacaagtgtcaagagcatt
tgaccaatttatcttcctgtgtaaaacccactgcattctacagagctgtgtaatgtggtttttttttttttgctctaatcagcttgaatttgccgccctc
atagctacacagcagctaatgtatcaaaactatagatttgtttttgatgggaacgcaccattatttcagtgtacataaaattacatataaaactaggaaa
agatcgcaggaaaagatttccaccctccattgacattcagggctgaatcatctgatatggaggtagaaacaatagaagtcctacctccacctgccaattc
agcccccaaatgtaagattttgctcttggcgcctttcacagagcgtggattgattagggattttaaatttactaaaatgcttgtggatagggggctaaaa
attgctttaatgta
3' EST: CF785449
[+]tttttttttttttttgtattgcaaacaagtacaaatttattttgttttgatattacttttatagtagataggtaaacacatcccattatgcttacgtagc
atgggtgggcacatatgccttacagttattagcaaataacagaaattcattaaaatggctactgaagcttattatggtttcttagccttgaaaataagct
gagatacccaaggccagagcaacttggacacagacaagataatgaggataatcataacaattgaagtaacacatacacaggccttcatgattgaaaacaa
aattgtgtaaattcaacattaagcaatttagcccctatcacagcatttagtaatttaaatccctatcaatcacgctctgttgaaggcgccagagcaaaat
ctacatttggggctgaattggcaggtggaggtaggacttctattgtttctacctccatatcagatgattcagccctgaatgtcaatggagggtggaaatc
tttcctgcgatcttttcctagttttatatgtaattttatgtacactgaaataatggtgcgttcccatcaaaaacaaatctatagttttgatacattagct
gctgtgtagctatgagggcggcaattcaagctgattagagcaaaaaaaaaaaaaccacattacacagctctgtagaatgcagtgggttttacacaggaag
ataaattggtcaaatgctcttgacacttgtaaaaagattacaaaccttatttgtggactattaatgtattttactattttctccctttgataacactcag
cagaacaaagaaatgactgctg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: