XB-CLONE-4350510
Clone Name: IMAGE:7025682
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Gene:
ppp1r3c
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785479 [+]
taattaatcccccccccccccgcccaatgtttcagcggctgcagcggagcgagtgtcactttctgagctgctcctatccagaatgatacagattttggaa
ccccgctctctcccacgatccataatgcctgtagatgtggcagttaggatatgcctagcacattctccaccgctaaaaaaatttctcagtccccctgatt
gcaggggaagaaattttgtgaaccgatataagccactaagaccatgcctcagtctgaaaaaggaatctgatttcaggagcaacgagtggaaccgctcaaa
gtctagaaacaaaaagaaggtcatctttgctgattccaaaggactttccttaacttcggtgcatgtcttctcagagtttaaggatgaatcttcattagat
cttcagtttgacctgatagacctagaagatataacagccagtttaaagctgcatgaagagaagaacttgatactgggattcactcagccttcagctgatt
atctgcagtttcgcaatcgccttcagaagagtttagtctgcctagaaaattgcagccttcaggaaagatctgttgcaggtactgtcaaagtaaaaaacct
gagctacaagaaatctgtcaagattcgtatcaccttcaacacctggaaatcatttgtagatgttgattgtgtctacatgaacaacgtctatggaagcact
gacagtgacacattttccttcgtcattgacataccccccaacattcccagtcatgagaaaatcgaattttgtatatcctatgaa
3' EST: CF785480 [+]tttttttttttttttcattatacaaaacataatttattattgaaagttcacatttacgtaaaacaggttaatttttacagtgataaaacattgtaggaaa
acagactgttgggttagaactcactctctcatcactctaaagttcaacatttgacatactgtgctaataaagtgacctttatcattccttaaagctgatt
tcgtttcacacattggatacagcaatatccaattaatctttatcagttttaaaaagcatcacagttcatttcacagtcaagaacgctgctcatttttttc
attctgacaccaaaaaacaatattgtcagcttacgaaggtactgtacatcaaaaaatacacatttaagccagttacaaaatagaaaaatacactgctcag
ctatttcatgtaaaaagtgcaagagtttctattgagagaaaaaggaagaatagagaaaataaaacataattctctgcgccataaaggtatttcataaaaa
gctgtcattactcttcagaaggaaaataaggtttgactatctgatgtgcaaaaatatcgactacaatcatttcatcctttaaggaacctacaattctatc
aaataccaaattcacatataaaggtgcaaaagcgatttgcgttaccttcaatatgaaggaaattgaattttctgacttctgtaattatctcaaaaaacat
aaaaagacggggaatattacagattgcttacaaacaagtgcattttttttttccgggcagggaaaatgagggtattattatcctgaaaaggtga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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