XB-CLONE-4350502
Clone Name: IMAGE:7025679
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Gene:
hes7.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785485 [+]
taattaatcccccccccccccgacacaagcgactttctcctcacacagagcagatgcattcccctgggatttgcccccaagctgctggctgcaggatgag
gaactcctaccccaaccgggaagataagaggctaatgaagccagtaatagagaaaagaaggagagaccgaattaatcagagcctggaacatctaaggacc
cttctcttggaggccactcatgatgagactttaaaaaaccccaaggctgaaaaggccgatattctgaagaagacggttcactttctcaaaatgtgtcata
atccagtgccgtcggatggaaagaaattgctgtcggggttcaaaggaggctttcgggaaggactcaaccaggccacctcctttctgaactcggctgacag
tatctgccagaagaagaaggagtacgtggtgcagaggctgtgccaacacatggagcagcagactcagaagcactgccacgactccgctcaagatgtctcc
tccagggtcaaccagaggcagatcttgccgagcccaccattgatctccagggttacagggaatggactggaacaaagccccgagaaccagaccagccgac
ctcctcattccaccatccatcttcatccagtttccttcaaaacgccctggtatggggccagaacagcaaccagacacccccccaaaacttttcaaggcca
aaccccaacaaaaaagccaaacccaagggacttctcttccccccgggacaagctccttttggttaaactcaccccccttggggggggaaggcccctgggc
ccataaaattattttttgtattttattcccccttccctttttttcccccga
3' EST: CF785484 [+]ttttttttttttttttttttttttttttttttacaaaacaacacctatagttctttaatttatagagctatgcacgtagaaaatacagagtgatgaaagt
ataaaaaagataaagatataatttaattttacagtccatatgtacaaaaaaagggggagaagggaggggataataaataatatttatggccagggcctcc
acacaagggctgaattaacaaaggagctggtcggggggaaaaaagtcctttgggttggctttttgttggtgttggcttgaaaagtttgtgggggtgtctg
ctgctgttcttgccccatacagggcgttctgaaggaactggatggaaatggatggtgggaatgaagaggtcggctggtctgggtctcggggctttgttcc
agtccattccctgtaaccctggagatcaatggtgggctcggcaagatctgcctctggttgaccctggaagagacatcttgagcggagtcctggcaatgct
tctgaaactgctgctccatgtgttggcacagccttctgcaccacgtaatcccttcttctttcgggcaaaaaactgtcacccaaaattcaaaagggagggg
gcctgggttaaatcccttcccaaaaaacccttctttttaacccccgacagcaaattttcttttccctccccaacgggcactgggatttatgaccaccctt
tttgaaaaaaggtggaaccgctctctctctacaaaaaaaatccgcccttttttttcaccctaggggggttttttttaaaatcctcaatacaagagggtgg
gcctctcaaaaaaaaaagggcccccttaagagatttccccgggcccccttaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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