XB-CLONE-4350463
Clone Name: IMAGE:7025662
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Gene:
glul
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785509 [+]
taattaatcccccccccccccgctcagtgggtttgtcaacaacttgctgagggaccagagggccagtgctgcttcccttgctaataagatcagccgccgc
catgtccgtctcccacagttcgagactcaacaagggggtgagggaacagtacatgaaactgccccaaggagggaaggtccaggtcacctacgtctggatc
gacggcaccggggagggagtgaggtgcaagacccggactctggatcaggaacccaaaaccatagatgaaatcccagaatggaacttcgatggatccagta
cgtaccaagcagaaggttcaaacagtgacatgtatctcgtcccagtccagatgttcagagaccccttctgcctggaccccaataagctggttatgtgcga
agtcttaaaatacaaccgcaagtctgcagagaccaacctccgacacacgtgtaagaagatcatggaaatggtgactgaccatcgcccatggtttggaatg
gaacaggaatacaccctgctgggcattaacgggcacccgtatggttggccaaaaaacggcttccctgggccaaaagggcccctattactgcgggcgtggg
gggccgaataagggttacggggcggggaaatccctggaaaggcgcattaaaagggggtgttctattacgcccgggcatttaagatcctgtgggcaccaaa
cgcggaaaattcatggcccttcccagtggggaaattcccggggggggaaccggtgcggaaggcattccaccagtgggggggaaccaccatattggaagtg
gcccagtt
3' EST: CF785510 [+]gatgctggcaccccggttggcgactccggccgagaactcgtggatactggacgtctcgtgctgcccggtgagtctccgggagttgtccttcccgccccgg
gggtcatacacgcagatgtggtagttgtgcctcttcccgagcttctcgatggcatcttctatgtgtttgagtcccccttcgaccctcatgctctctgtac
tgtagttggtatggcaaccagcgccgttccagtttccggtcatgggcttggggtccagcgtggccaccaccccgaagtcctcgcacacccggtggaggat
gaacctggccatccataggtggtcccccatgtcgatgccttcgcacggtcccacctggaactcccactgggagggcatgacttccgcgttggtgccacag
atcttgatgccggcgtatagacacgccttgtagtgcgcctccacgatgtcccgcccgtacaccttatccgcccccacgccgcagtaatagggaccttgtg
gcccagggaagccgttctctggccaaccatacgggtgcccgttaatgcccagcagggtgtattcctgtttcattccaaaccatggggcgatggtcagtca
ccattttcatgatcttcttacacgtgtgtcggaagttggtctctgcagactttgcgttgtattttaagacttcgcacataaccagcttattgggggtcca
gggcagagggggtctctgtaacatctgggactggggacgagaaaacatggtcccctggttttgaaacctttcttgctttgggtaccntacctgggatccc
atccaaagattgccctttctttgggaata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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