XB-CLONE-4350411
Clone Name: IMAGE:7025892
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Gene:
rps11
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077050
[+]
GCTTTCCCATCCAGCAGGCAAAGATGGCGGATATTCAGACCGAGAGAGCCTATCAGAAGCAGCCGACCATCTTCCAGAACAAGAAGCGGGTGCTGCAGGG
TGAGACCGGCAAGGAGAAGCTCCCACGGTACTACAGGAACGTCGGCCTGGGGTTCAAGACCCCCAGAGAGGCAATTGATGGCACCTACATTGACAAGAAA
TGTCCCTTTACTGGCAACGTGTCGATTCGTGGGCGTATCCTTTCAGGAGTGGTGACAAAGATGAAGATGCAGAGGACAATAGTGATCCGCAGAGATTACC
TGCATTACATTCGCAAGTACAACCGGTTTGAGAAGCGCCACAAGAACATGTCTGTGCACCTCTCCCCCTGCTTCAGGGATGTTCAGATCGGAGACATTGT
CACAGTCGGAGAATGTCGTCCCCTTAGTAAAACTGTCCGCTTCAATGTCCTGAAGGTGACCAAAGCGGCCGGAACCAAGAAACAGTTCCAGAAGTTCTAA
GGTTCCTTCCTGTCTGTTCCAATAAAGCTTCTAAGAGTTAAAAAAAAAAAAAAAA
5' EST: CF785542
[+]taattaatcccccccccccccgctttcccatccagcaggcaaagatggcggatattcagaccgagagagcctatcagaagcagccgaccatcttccagaa
caagaagcgggtgctgcagggtgagaccggcaaggagaagctcccacggtactacaggaacgtcggcctggggttcaagacccccagagaggcaattgat
ggcacctacattgacaagaaatgtccctttactggcaacgtgtcgattcgtgggcgtatcctttcaggagtggtgacaaagatgaagatgcagaggacaa
tagtgatccgcagagattacctgcattacattcgcaagtacaaccggtttgagaagcgccacaagaacatgtctgtgcacctctccccctgcttcaggga
tgttcagatcggagacattgtcacagtcggagaatgtcgtccccttagtaaaactgtccgcttcaatgtcctgaaggtgaccaaagcggccggaaccaag
aaacagttccagaagttctaaagttccttcctgtctgttccaataaagcttctaagagttaaaaaaaaaaaaaaaag
3' EST: CF785543
[+]tttttttttttttttaactcttagaagctttattggaacagacaggaaggaaccttagaacttctggaactgtttcttggttccggccgctttggtcacc
ttcaggacattgaagcggacagttttactaaggggacgacattctccgactgtgacaatgtctccgatctgaacatccctgaagcagggggagaggtgca
cagacatgttcttgtggcgcttctcaaaccggttgtacttgcgaatgtaatgcaggtaatctctgcggatcactattgtcctctgcatcttcatctttgt
caccactcctgaaaggatacgcccacgaatcgacacgttgccagtaaagggacatttcttgtcaatgtaggtgccatcaattgcctctctgggggtcttg
aaccccaggccgacgttcctgtagtaccgtgggagcttctccttgccggtctcaccctgcagcacccgcttcttgttctggaagatggtcggctgcttct
gataggctctctcggtctgaatatccgccatctttgcctgctggatgggaaangcggggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: