XB-CLONE-4350394
Clone Name: IMAGE:7025885
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Gene:
csnk2b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077003 [+]
GCTAATGCGCTTGCGCACTAAGGGGACCGGGCCTAGTGCGCTGCGGTGAAATTTTGCCGGTTAAAAGCCTCCAAATATACGGCAAGCAGCGGTCCCTTGG
AGGCTAGAAAATGAGCAGCTCAGAGGAGGTGTCCTGGATTAGCTGGTTTTGTGGTTTAAGAGGCAATGAATTTTTCTGTGAGGTGGATGAAGATTATATC
CAGGATAAGTTTAACTTGACAGGACTCAATGAGCAGGTCCCTCACTATAGACAGGCACTGGACATGATTCTGGACCTTGAACCTGATGAGGAATTGGAAG
ATAATCCCAATCAAAGTGACCTCATTGAACAAGCCGCAGAGATGTTATATGGACTGATCCATGCCCGTTACATACTGACTAACCGTGGCATTGCCCAGAT
GCTGGAGAAATATCAACAGGGCGATTTTGGCTACTGTCCCAGAGTATACTGTGAGAACCAGCCCATGCTACCTATTGGTCTTTCAGATATTCCTGGGGAA
GCTATGGTAAAGCTGTATTGTCCTAAGTGTATGGATGTTTACACACCCAAATCCTCACGCCATCATCACACCGATGGAGCATATTTTGGCACTGGATTTC
CTCACATGCTCTTTATGGTTCACCCTGAGTATAGGCCCAAGAGGCCAGCCAATCAGTTTGTTCCAAGGTTATATGGCTTCAAAATCCACCCTATGGCATA
CCAGCTTCAGCTACAAGCAGCCAGCAACTTTAAAAGTCCTGTGAAGACTATGCGTTGAGTATTGCCCATTAAATGGTTTATACATGGGGTCTGCACACCC
CATAAACCTCATTTCCTTCCTTCCATTGACAATGAAAAATGTTTGTTTCAGTGTATTATTTGCTCACAATTTTGATTTTAGTCCCGTTTAAAGTGTATTA
CTGCAATTTACGGTTGTGGTTAAATAAAATTATAAAGAAAAAAAAAAAAAAAA
5' EST: CF785554 [+]taattaatcccccccccccccgctaatgcgcttgcgcactaaggggaccgggcctagtgcgctgcggtgaaattttgccggttaaaagcctccaaatata
cggcaagcagcggtcccttggaggctagaaaatgagcagctcagaggaggtgtcctggattagctggttttgtggtttaagaggcaatgaatttttctgt
gaggtggatgaagattatatccaggataagtttaacttgacaggactcaatgagcaggtccctcactatagacaggcactggacatgattctggaccttg
aacctgatgaggaattggaagataatcccaatcaaagtgacctcattgaacaagccgcagagatgttatatggactgatccatgcccgttacatactgac
taaccgtggcattgcccagatgctggagaaatatcaacagggcgattttggctactgtcccagagtatactgtgagaaccagcccatgctacctattggt
ctttcagatattcctggggaagctatggtaaagctgtattgtcctaagtgtatggatgtttacacacccaaatcctcacgccatcatcacaccgatggag
catattttggcactggatttcctcacatgctctttatggttcaccctgagtataggcccaagaggccagccaatcaatttgttccaaggttatatgggct
tcaaaatccacccctatggcatacccagcttcagcttacaagcaggccaagcaaactttaaanaagtccctgttgaagaacttatgccgttttgaa
3' EST: CF785553 [+]tttttttttttttttctttataattttatttaaccacaaccgtaaattgcagtaatacactttaaacgggactaaaatcaaaattgtgagcaaataatac
actgaaacaaacatttttcattgtcaatggaaggaaggaaatgaggtttatggggtgtgcagaccccatgtataaaccatttaatgggcaatactcaacg
catagtcttcacaggacttttaaagttgctggctgcttgtagctgaagctggtatgccatagggtggattttgaagccatataaccttggaacaaactga
ttggctggcctcttgggcctatactcagggtgaaccataaagagcatgtgaggaaatccagtgccaaaatatgctccatcggtgtgatgatggcgtgagg
atttgggtgtgtaaacatccatacacttaggacaatacagctttaccatagcttccccaggaatatctgaaagaccaataggtagcatgggctggttctc
acagtatactctgggacagtagccaaaatcgccctgttgatatttctccagcatctgggcaatgccacggttagtcagtatgtaacgggcatggatcagt
ccatataacatctctgcggcttgttcaatgangtcactttgattgggattatcttccattcctcatcangttcaaggtccagaatcatgtccagtgcctg
tctatagtgagggacctgctcattgagtcctgtcaagttaaacttatccctggatataatcttcatccacctcaaagaaaaattcattgcctt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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