XB-CLONE-4350391
Clone Name: IMAGE:7025884
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Gene:
h2az1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785555
[+]
taattaatcccccccccccccgttttcgagtgccagagcgccagtttgggtttaaggaggaattacaggacttgattaagataaaaatggctggtggcaa
ggctggcaaagataccggaaaagcaaaggctacctctatcacccgatcctccagagctggattgcagtttccagttggtcgtatacatagactgctgaag
aacagaactaccagccatggtcgtgtgggtggtacagcggcagtatatacagctgctatcttggaatatttgactgctgaggttcttgaattggctggaa
atgcttccaaagatcttaaggtgaagcgtatcagcccacgtcacttgcagcttgccatcagaggtgatgaagaattggatgctcttataaaagcaaccat
tgctggcggtggagtcattccacacatacacaagtcactcattggaaagaaaggacagcagaagactgtttagtcaatgtcagaaacccaggaactcgtc
agacttgatcagccttctttctgaagaaaagactggttttcacactcatccaagactgagattcccatttcaaagcagatgcttggggggttttaatgtg
cccatgggcttccaagaagccagtttctaatggactctggtttttacatacagggttttttttatgccaattttttggggaaaagtataaacatggaaac
acctgaagcttgattttgtttaagaaaatgaatttaaataaatgtttgggcttaatgggtaatgtcctggtaaatgcctttttattttccagccg
3' EST: CF785556
[+]tttttttttttttttttttacaaccacaaaatcgcagtattttaataccatgatttaacacaacaggagaaacatttcaaaatctagtattgcacagatg
gtctatttcagcttaacagctagaaataaaatgcaattacatgacatacccattaagccaaacatttattaaattcatttactaaacaaatcaagcatca
gctgttccatgtttatacatctcccaacaacttgcataaaaaaaacctgtatgtaaaaccagagtccatcagaactggcttcttggaagccatgggcaca
ttaaaaccaccaagcatctgctttgaaatgggaatctcagtcttggatgagtgtgaaaaccagtcttttcttcagaaagaaggctgatcaagtctgacga
gttcttgggtttctgacattgactaaacagtcttctgctgtcctttctttccaatgagtgacttgtgtatgtgtggaatgactccaccgccagcaatggt
tgcttttataagagcatccaattcttcatcacctctgatggcaagctgcaagtgacgtgggctgatacgcttcaccttaagatccttgggagcatttcca
gcccaatcaagaaccctcgccagtcaaatattcccaagataccagctggtatatactggccgctggtaccccccccacgaacccaggggctgggaaagtt
ccgggtctcctttagccagtccttatggtttacccaaacccaacttggggaaaactgggcaaatcccaacctccttggggaggagaaccggggggtgatt
aaaaaggggtagaaccctcttttggccttttttccccgggggataccctttttggtcccccaacaccct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: