XB-CLONE-4350379
Clone Name: IMAGE:7025879
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Gene:
tpt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785563
[+]
taattaatcccccccccccccccttttctttccccaccctccgccggcactgatcctccctgtgtgctccggcactacaacacccaggccgccaacatga
tcatctacaaggacaccgtcactgaggatgaaatgttctccgatatctacaagatcgccgaaacccccgacggcatgtgttttgaagtggaagggaagat
catccaaagagtagaaggtgcaattgatgatgcgcttattggtggaaatgcttcagctgagtgccaggaggaggacatcggtggtgcaacaacagtcagc
ggggtggacatagtgattaatcacaaacttcaagaaactggtttcaccaaggactcttacaagaattacattaaggattatgtaaaactagtcaaggcca
aacttgaggagactgatcccgacagagtaaaacccttcatgaaaggaattcaagatagagtaaaactcatacttggaaacttcaaaaactatcagtttta
cactggagaaagaatgaacccagatggtatggtggctctgctagattaccgtgaggatggtgtaacaccttttatgattttctttaaggatggcttgatc
agtgagaaatgttaacattcaatttggttcttccatcttggctgctgcttttcatccatgaacaccagggtctaagcaagatcttactgaactcttaaag
gactgatgatacatttgagctttgttccttcaatggcccctaaatttattcaaagtgggaggctttttttgggttttttttaaattgaaaaatccctttt
ggtccattttagaagtaaaac
3' EST: CF785562
[+]tttttttttttttttcattgtttaatcttgtttttatttaggatcagtaagaaaaaaagggaaagatcttctgtgatggtagagcctgttaacaggcaac
agtcaatgttccaaatccaaacatgttcttgtgcaaacaacctgcagtgtaaccaaccccttcacagccagaggtatttgcaacactgttgaaaggcctt
ctggcagtggaggagtctaaaaacctgaagccgagaggcagattgaatactttggctgtttccttaagtgtgcagattgtccacaaactgaaggtccgct
tcttacaaaatgttctggcagtcagtctgataagtcaaagcttttgcgcaacaaatcttaaatgcacaagacccagtcttcaaaagtaattgcagatcaa
accaagaggcaatgtctacagtggagaattatgggcttttatttactctaaatgacaaaaggatttcaattaaaaaaacaaaaaagcctccacttgaata
atttagggccatgaagaaacaagctcaatgtatcatcagtcctttaggagtcagtaagatcttgcttaaacctggtgttcatgaatgaaaagcagcagcc
aagatggaagaaacaaattgaatgttaacatttctcactgatcaagccatccttaaaaaaatcataaaaggtgtttaaccatcctcacggtaatctagca
gagccaccataccatctgggttcattctttctccagtgtaaaacggaaagtttttgaattttccaagttggagttttactcctaccttgaattcctttca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: