XB-CLONE-4350374
Clone Name: IMAGE:7025877
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Gene:
fdxr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785566 [+]
taattaatcccccccccccccgtcagtgtgtgtatgagccccatgttggtaggatgtgggggctgtcccgctgcctgagagttccccggggacggctgct
gctgcttcctgccggggtgcaaaggtccttctcctccgtctctcccactcctcacatctgtatcgttgggagcggcccggcggggttctacacagcccag
cacatcctcaggcacaaccctcaagctcaggtcgatatctatgagaaactgccagttcccttcggcctggtgcgctttggcgtggcgccggatcacccag
aagtaaagaatgttattaacaccttcacgcagacagctcggtcggagcgctgtacattcctgggcaatgtgacggtgggccgggatgtgacagtggagga
gctgcagggggcctatcacgctgtggtgctgagttacggtgctgaagacaaacgtgagctggggatccccggagaggagctgcccggagtccattctgcc
agggactttgtgggctggtacaatgggctccctgacaacagacatcttcatccagacctgagctgtgacacggcggtgatattagggcaggggaacgtgg
ctctgggatatgctccgattctcctttccccagtcgacttcctggctaaaaacagacattacccaaaattgccttgggaggttctgaacccaaaaacccc
ctttcctgatggggtgtggaatgggtccggggcccccgttggggcccctctgccaagtgggccatttttacccattaaagggtatttttctcgggggnaa
taatttaaaaaaaaacctttaacccaggcaaaaaaatttttgggaccccaacaaagtcttttgtta
3' EST: CF785565 [+]ggctgctctcttcccatcctcactgggtaacacggccagggggctccttaggaagcgcaagccccactctctctcagcctgggcccaattggctgccacc
ttctggccaggcttctccagtgcagctttcactagcagctcggtgaggcgcttccttggccgagggaggtctttgcttgccttctccaaacccacataat
cagcggggtccagctttggccgagtctctggtaagtttatcatctcccgcaactcctttatggtaaatgccacttgcagaggcccacggcgcccgaccat
ccacaccctccgtatgcggctttggctcagagcatccaaggcagtctgggtaatgtctgtttttcgcaggatgtcgactggggaaaggagaatacgagca
atatccagagccacgttcccctgccctaatatcaccgccgtgtcacagctcaagtctggatgaagatgtctgttgtcagggagcccattgtaccagccca
caaagtccctggcagaatggactccgggcagctcctctccggggatccccagctcacgtttgtctttcgcacccgtaactcagcaccacaagcgtgatag
gcccccctgcaagctcctcccctgtcacatcccgggccaccgttcaatttgccccaggaatggtacaggcgctccaaccaagctgttctgccgtgaaagg
tggtaaaaacacttctttaactctggggggaattccggcgccaccccaaaaccccaccaaggcccaaagggaactgggcattttccctcaaaaaaatcca
ccctgaacctttggggggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: