XB-CLONE-4350371
Clone Name: IMAGE:7025876
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Gene:
naa50
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084533
[+]
CCCCCCCCGCGTGGTGCAGGTAGGGGAAGGCGGACTGCGTGAGGCACCGAAGTCTCCTCAGGAAAGGGCTCAGCGCTCCCCAGGCTGGCGGCTTCCCCGA
AACCCCCCGACAACGCGACTCCTTCTGCCCCCTCCCAGGACTGCCCCATTCTCTTCTTGTCAGCGCACCGACACGCCTGCCCCGGTCATACAGCGAGGGA
GTCCGGCGGCCCCGGGACTAGCGCCCCCTGCAGAGATGAAAGGCCGGATCGAGTTGGGCGATGTGACTCCACATAACATAAAGCAACTGAAGCGGCTGAA
CCAAGTCATCTTCCCAGTGAGCTACAATGACAAATTTTACAAGGATGTGCTGGAAGTCGGAGAGCTCGCCAAACTGGCTTATTTCAACGACATTGCTGTG
GGTGCAGTGTGCTGTAGAGTGGATCACTCCCAGAATCAGAAACGACTATATATCATGACCCTGGGGTGCCTGGCCCCATACCGCAGGCTTGGAATAGGAA
CAAAAATGCTGAACCATGTTTTAAATATTTGTGAAAAAGACGGCACCTTCGACAACATTTATCTCCACGTTCAGATCAGCAACGAATCCGCCATAGATTT
TTACAGGAAGTTTGGCTTTGAGATTATCGAAACCAAGAAGAACTATTACAAGAGGATAGAGCCGGCCGACGCTCACGTCCTGCAGAAAAACCTAAAGATT
TCATCTCCGGGGCAGAATGCAGACGTGCAAAAGAGCGAAAACTGAACGGAAAAAGCAACAAACACTTCTCTTGCACTTGCTCGTCGCCAAATACGGAAAG
AGACCCATTGAGTTTTATTCTAAACGTTCACCTCTAACATCCCCCCTTCTTTCTCCACTTTAAAAATGATAAAAAAAAAAAAAACAGAAAAAATTGCTTC
TTCCAAGAGTTACACAATCTGTCTCTCTCACACAAACAGGTATTTCAGTCGAACTCGGAATATTTCCCCGGGGCAGAGCTCAGTTCGGCGGGGGAATGTG
GATTTCTTTGGCTTTACAAGTTTTTTTGTTTTTGGTTTATTTTTTCCCCCCCTTTTGTTTGGGAGAGTGAGTGGCTTCTGTCGGTCTGGAATAGTCTTTA
AATAAAATCTCATTTCTTTTTAGCAAAAAAAAAAAAAAAA
5' EST: CF785567
[+]taattaatccccccccccccaacccccccgcgtggtgcaggtaggggaaggcggactgcgtgaggcaccgaagtctcctcaggaaagggctcagcgctcc
ccaggctggcggcttccccgaaaccccccgacaacgcgactccttctgccccctcccaggactgccccattctcttcttgtcagcgcaccgacacgcctg
ccccggtcatacagcgagggagtccggcggccccgggactagcgccccctgcagagatgaaaggccggatcgagttgggcgatgtgactccacataacat
aaagcaactgaagcggctgaaccaagtcatcttcccagtgagctacaatgacaaattttacaaggatgtgctggaagtcggagagctcgccaaactggct
tatttcaacgacattgctgtgggtgcagtgtgctgtagagtggatcactcccagaatcagaaacgactatatatcatgaccctggggtgcctggccccat
accgcaggcttggaataggaacaaaaatgctgaaccatgttttaaatatttgtgaaaaagacggcaccttcgacaacatttatctccacgttcagatcag
caacgaatccgccatagatttttacaggaagtttggctttgagattatcgaaaccaagaagaactattacaagaggatagagccggccgacgctcacgtc
ctgcagaaaaacctaaagatttcatctcccgggccagaatgcagaccttgcaaaaagagcgaaaaactggaacgggaaaaaagccaacaaacactttctc
ctttgcaccttgccttcgttccgca
3' EST: CF785568
[+]tttttttttttttttgctaaaaagaaatgagattttatttaaagactattccagaccgacagaagccactcactctcccaaacaaaagggggggaaaaaa
taaaccaaaaacaaaaaaacttgtaaagccaaagaaatccacattcccccgccgaactgagctctgccccggggaaatattccgagttcgactgaaatac
ctgtttgtgtgagagagacagattgtgtaactcttggaagaagcaattttttctgttttttttttttttatcatttttaaagtggagaaagaagggggga
tgttagaggtgaacgtttagaataaaactcaatgggtctctttccgtatttggcgacgagcaagtgcaagagaagtgtttgttgctttttccgttcagtt
ttcgctcttttgcacgtctgcattctgccccggagatgaaatctttaggtttttctgcaggacgtgagcgtcggccggctctatcctcttgtaatagttc
ttcttggtttcgataatctcaaagccaaacttcctgtaaaaatctatggcggattcgttgctgatctgaacgtggagataaatgttgtcgaaggtgccgt
ctttttcacaaatatttaaaacatggttcagcatttttgttcctattccaaagcctgcggtatgggggccaggcaccccaaggtcatgatatatagtcgt
ttctgattcctgggagtgatccactctacagcacaactgcacccacagcaatgtcgttgaaataaagccagttttggcgagctctccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: