XB-CLONE-4350364
Clone Name: IMAGE:7025871
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Gene:
thumpd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785572 [+]
taattaatcccccccccccccgggaaccagctgcggccatgctctcgcagtaacacggagataacgtgcgggctgcgtgtagaagagacagataccagga
atacacatctaggtgaaggctgtaactgttgttagcaccaggaacttttttattattccacaccaatcatgagcacagatgggcaaaagggcaagaaaag
atataagtctcaattctgcggcaatgccaagaggcagaagcggcgccatgagctggaggttggaatgcaagggatccttatcacctgtaacatgaatgag
cggaaatgtgtagcagaagcctacagcctactgaatgaatatggggatcagatgtatggtcctgagaagctaagtgagaaagacgatgggctatcagaaa
gtgaggaagaggatgatgatgcagaagcagctctaaagaaagaagaggatcagattcgcacttcaacagaaaaaaacctgccgtagattttcgtcttgtg
gaaagtggtaccatacaatgtttattttttattaaaaaccactgaaattgtggaagccatggaaaaaacttggggggcatccaatatttttttgaaaaag
gacggtgcctaccacctcccccctaggtaatagaaactaaacggacacccgggggggttccaatacccctcccccggcaatggacattgcccccggtgtt
ttctcgggagtaaccattgctcaaagggcgttattttctctttggaagggaaccttttggatagaaacatatatctcgctgtggaataacactatttttt
ttttagccaacctccctgtgtagnttaataaaatccttcccctcaaaaccaagggggaaaaccaccttttctccaaaatttgggcccttatttaataggc
gcgcggggataaatcaatactaaacccacagttattcgttaattatcaagagcttctgaaaaatttgggcaaatatcatcgggacaacctatattccacg
aggtggaattttcttccatccaaatgn
3' EST: CF785571 [+]tttttttttttttttcttttttgccatttcactaaaatttatttacaatccaaaatgtttagaaaccaatactaaattgcacaccatagcaattagactc
aaagtttttggaattcgaattgttaaaagagaaactgaaacatacatccatggcctaatggtttttttaaccgaccattaaaataaaaacaagtatttct
ttagtatggtatgaattttacaaatggtatacactgcactacccccaaaagccctacatactagaatgcatgaaatctcccaccagaccatccgtataca
gcacctggctataaactaattgcaacttcagtctcaaaaagtccacagggttcatcccagagaataaagaacggaattaaaccattccagttctactgac
ctacttcggctaaaagcacaagtagcacaaaatagtaggcaggagttgctcctgcaaggtacacatcaatctttgccgagataggtggggtgggctttaa
tgcagacattacacatttgcatcatatgtgttgtgcttatgataaacaagaacagcagtttcctgtaagagtgatggcacaagggatgttttgctgcctg
cactaaatctcctacaatgcagataacaaaactactgaatataccttttgtattgcagtcaaatgtactcactgccaaactgcaggatttacggtgggtg
actaaaaccttctctgtgccatcacccttaaaaggcagtgtactgtagctcaaacaccgtaaaacataaaagtgagtgtattgaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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