XB-CLONE-4350358
Clone Name: IMAGE:7025869
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Gene:
bambi
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785576
[+]
taattaatccccccccccccccggtgcgctgagccgcaggcagctgagtgtactgtgcgactcgcagggattacaatagccgctgccgctgtccgtgttg
ctttactgtggattttgggaagctgatcccgcagagcaggggttattccccaccgagagccattcctcgcccactagcgaagggttcggcgcttcttccc
tgggctctggatgctaaagctgggctggatggtgggatctctctggcgctgaagacgtgcaagcctgcccgcagggacctttaaatggatcgccaagcca
gcctgatatctctctggcttcagttagagctgtgtgccatggccatcctactaaccaagggggaaatcagatgctactgtgacgctccacactgtgttgc
gactggctacatgtgcaagtcggagctgaatgcctgcttctcaaggctccttaacccccagaacacaaactctcctctcacccacggctgcttggactcc
ctgaccagcaccacggagctgtgcaaggcaaacaccgagcagaatcacacaggcgcgccggcgctgaggttggagtgctgtcacgaagatatgtgcaatt
acggaggtctgcaccatgttctgtctcatcccaaggaatgatacatcaaggtacaggaagcagaacacccaactggacaacccaagggaacccttcttcc
cgaaaggtcccacgaaattgaaccctcctctccaaagaaccttgtggtttacaggggcacccttgttcataacctggtggcccattaatcttgggggggg
aactgattccctggggggggcatcccttcaataaaaggcctgtggcccctctttcgaaa
3' EST: CF785575
[+]aaaggccccctactggaccattgtctcaggaaggcggtcatacaaattccagcttgccgtgtccgctgtacatcccccaatggaccaatgagatgatttt
gtcattgctaaggtccgtgtgcctcagtttgtcacaggtcaggcagcagttttcgtgaccagtcactggcaccatgcactccaagtctaatttggccatc
tggcccttttttgaatggtggccgtgaaagctgtagtgaaaccgcgacagcatctggtgtctctgatcttttagtcttttgtttcccctgcccagcaatt
cgaatggcaagctttttagggacaaccgggattcattccccccacctatgggcccaaccttttaaaagcttccccctaaacccaaatttctttggaagaa
aggcaattcctggaaaccttccggaaaaagggtcccctggggtttccccctgttggggggcccgccccccccggaaccccaaaaaaacaccccccccggg
gaaaaaaaaaaaaaaaaaaatttgtgggaaaaaacccttttgtatttttgcccaattattttttttttagaaaaaaatcctttcccacctttttttcccc
cccggggggcccccttccgggggaaataattcccccccccggggggttttttttttttttaaaaaaaacacccccgggggggggggtgcctttcgggggn
tctctcccccccccccccccccgggaagggagaaaaaaaaaaaaaaattttttttttttttttctcggggggggggaaaaaaaaaaaccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: