XB-CLONE-4350355
Clone Name: IMAGE:7025868
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Gene:
mpo
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785577
[+]
taattaatcccccccccccccgacaaagagaagtggcaaggaactacaaaaggatggcacctctgtacatcagttttgggttcctactgattcttggatc
ggtgcatgtcagtttatcttccttctacgatggcgtggaagagctggacaatgatataattctcaactcggttcaggaggcaaaaaagctggtggatact
gcttataaacgcacccgggatgtcctcaaggcacgcctaaaacaaaaaaatgtgcgtccctctgatgtcatggcgttctttaagcaaccggtagccacca
gcagaacccatatccgagctgctgattatatgggcaccacacttgaactcctttcatagaagattcggagtgtttataggagaccattccacgcccccca
tgcactgactgccaactaggcttgacacaatatataaggactcctggatgtatcttttcttaccgttgtaaagggcgcccgtcccaattagccgaagatt
cgcaccttttacaagggcgagttttaccaaccccggaggtttttcccccgctattgtgcgttctcctaacatgtgtcctctcagtagcgctcttgttttt
cacaatccaccagggacagtaattttattatgtcacgaaatccgcgtttttccaactgatcgatgtgtctggggcaccgcactatggtaacttctgcgca
caattaagaaagtgtataattttgtgaaaaatacacccatgcgtctgaacatcctctcttccagataggtggaagacgggtgggccccctggttttcacg
ctcactataacctcacagaaaactttttttgttgcagtagagaattttctccccccccgaaatatagagggtaaataaccgtgctgtttcccgcgagtaa
taataaatatccatcttaaaacgcttttccacacctcatcggtatctcaaatattttatacattacacgggtgtttttcgtcagtgtttatatcggctct
ccgcgttgtttttcttactcacattttagtcctggcccgtcgcctattattatattatgttgatggggctgtaatagatgcttttattcgacgagcgccg
ccacctcacggtt
3' EST: CF785578
[+]tggatcatttggtggaatccgtaaaggaaagcagggcagctcctttgccaactgtggtcacagtctattcccttcaagaagcttgaccttgcaggtgttt
caggtgccaagtcaacatcatgatcaatccactgaccccactgcataaacatgagggagcgttggttgtccagggtctgttccctttcaggaaattgcaa
aatctggttggatacagcccgtgctagaggcaacataaatccattgatccttcgattctctgtccacccgcggggaagggatctgctgtcctcatactgt
gctggaagcaggcgtgtgtagggcctgttggagacgccaaagttgggaaacctcctgttgttacactcccctgtgaaggtgcgatatcggctattgggac
aaggccttgacaaatgtaagaaagcacatccagaaaccttatatattgtgtcaagctggttggcagtcagtacatctgttatgttgaatggtctcctata
aacactctgaatcttctctgaaaggagttcaagtgtggtgcccatataatcagcagctcggatgtgggttctgctggtggctaccggttgcttaaagaac
gccatgacatcagagggacgcacatttttttgttttaggcgtgccttgaggacatcccgggtgcgtttataagcagtatccaccagcttttttgcctcct
gaaccgagttgagaattatatcattgtccagctcttccacgccatcgtagaaggaagataaactgacatgcacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: