XB-CLONE-4350352
Clone Name: IMAGE:7025867
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785580 [+]
taattaatcccccccccccccaaaaacctcctctctggctctgagtagagttcttgcttcaacagtgtttgaacggaaccctctctgagtcatttttaga
ccaaaatctctctctctctcttgcattactcttttttttgtgacttttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgcgtcag
aacttcaacagcgactgcgaagccgccatcaaccgtatggtgaacatggagttgtatgcctcctatgtctatctgtccatgtcttactatttcgatcgtg
atgacgtggcactccaccatgtggccaagttcttcaaggagcagagtcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaacgtgg
gggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaatggggtaacaccctggaagccatgcaggccgctctgcagctggagaagactgtg
aaccaggctttactggatctgcacaagctggcatctgacaagactgatcctcatctctgcgacttcctggaatctgagtacttggaggaacaggtgaatg
ccatgaaggagcttggagactacatcacaaacctgaagcgccttggggtgccgccaaaacggcatggggcgagtacctgtttcgacaagcacacccctgt
ggggaagaagtaggctaaagcgccacttcctcaggggtggggaaaacaaacccaagttcaggcctcctcctggtttttccaaaaaataacatggacctcc
ctttttaactaaatcccgtggtggtgggggaatttacaccaccccaattaaaggccttta
3' EST: CF785579 [+]tttttttttttttttggaatgctgaaaaaactttattgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttat
tcccatcatcctcaagataaaaaggtgtcaacaggggcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctg
actggttgtttccaccctgagagtgcgcttagctactctcccccagggtgtgctagtcgaacaggtactcgcccatgccgttctgcggcaccccaaggcg
cttcaggtttgtgatgtagtctccaagctccttcatggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttg
tcagatgccagcttgtgcagatccagtaaagcctggttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcac
gctctggtttcttgatatcctgaaggacggcgcggcccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctc
cttgaagaacttggccacatgggggagtgccccgtcctcacgatcgaaatagtaagaactggacagatagacatagggaggcatacaactccatggttca
cattacagtttgaggcggcttcccaatcgctgttgaaatttctaacgcacctggggattgcatggcggcgttttcggggttttgctggggggccaaaan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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