XB-CLONE-4350347
Clone Name: IMAGE:7025865
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785583 [+]
taattaatcccccccccccccagtctttgtgagtactccatctgcaggagggctcgcacatagaaaaaacaaatatatatatatataataagtgcaagca
agccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacacaagccctca
gtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggctaaaccagc
cccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttcctgcaaagggtaaaaaggga
gcaaagggcaaacagacagaggaagcctaacaaagaaaaaacctaagggaagaccagcccgtcctgaaaaacgggaaaaaccaaaaaagtggaccaaggg
ccccccggccctttcttgaatgggaagggcggaaaaaatgggaaaattccaaaaagttcctgtgaaaaaataaacaccccttttcacatcccctttggga
tacaccacggaaagagggcccctctcttattataaaaataccacagaagggggtggggtagtcttccccccccgggggggtaaatccacttcctctcccc
cccccatgtgggggggtagtacaaaaaatttttttctcccggcgaagagggggagagaattattacttttatatatttatgaggaaccacataagcccgc
tatattgttttttttgtgggggataaaatatgagctctccacaatgtgattttttctattatttctaataaattaacgcttttttctatataaatttctc
tccncggggcgaaataaaaacctcctcttgtctttttgtgttataaacagaaaatatgaggggagagggggaaaaaattctccccccctacacccaaatt
tatttttttttgtgggagagtgggacaaaacaaaaaaaaaaattattttattctgttgtgttataaacccacccttttatataggagatattttggggat
acacaatgtttggcccc
3' EST: CF785582 [+]tttttttttttttttattttttagagggaaatccgtcactcttgggagatttattagtttaaaagtgtaaattgtacgcaggacaacatgagtacaaaga
acagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgttcgaccggcagcctgcatagagtacta
gccgttttgtaacaaaataaaaaaaacattttagactgctcaaggcttgcactccccacagtaactggtttgtggtgtagtattaactggtatagtattc
atagcctttacaaaccatacaggggcaatgtaccaaatcataagtgtttaaaaaaaaattttttttacaacaaaaaaatggtggggattccctcctattt
taaaaatgtgttttctagaattaaactactaaaaaacttgcatttacaaaatagttgataaaaatattcctctgaattgtacaagggaggtacaggaacc
actgataaaagactcggtacaagatgaaggttattcagactttgattccttgtcgcctccatcagaagcaggggcctcgtcactttttgtttctccgttt
tcagacggctggtcttccttagcttcttctttgttagcttcctctgtctgtttgccctttgctccctttttaccctttgctggaaacttttttctccctt
ctcctcagggcttttcctttaagggtggcttggtcttttttttggcttttggttcagcttttggggggggctgggtttagcccaacaacccggcccaatc
tccctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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