XB-CLONE-4350341
Clone Name: IMAGE:7025863
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Gene:
rps8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785587 [+]
taattaatcccccccccccccgctctttctttccagccagcgcctgagaatgggtatctcaagggacaactggcacaagcgccgcaagactggtggcaaa
aggaagccttatcacaaaaagagaaagtatgaacttggccgtcctgctgcaaacaccaagattggtcctcgccgtatccacactgtgagagtccgcggtg
gtaacaaaaagtatcgtgctctgaggttggatgctggcaacttttcatggggttcagagtgttgcacacgtaaaactaggatcattgatgtggtgtacaa
tgcgtcaaacaatgagctggttcgaaccaaaacccttgtgaagaactgcattgtcctggttgacagcactccttacagacagtggtttgaggcccactat
gctctcccactaggacgcaagaagggagctaaactcacccctgaagaggaagaaatcctaaacaagaaacgatctaagaaaacacagaagaaatatgagg
agaggaaaaagacagcaaaaataagcccacttttggaagaacagttccagcaaggcaaactgcttgcatgcatagcctccagaccgggacagtgtggtag
agctgatggttatgtactggaagggaaggagttggagttctacctgagaaagatcaaggccaaaaaaggggaataaacatggttaccacactttatcaat
caaaataaatggttatttaaaaccaaaaaataaaaaaaaaaaggcatcccacctaagtttctaagaagcgggcccgcccacaccgccgggtggggaaacc
ttggggccccgttgggccccacaccttttgtttttaattggcgcaagccttttaatacattgagagttaa
3' EST: CF785586 [+]tttttttttttttttgttttaataacatttatttgatgataagtgtggtaacatgtttatttcccctttttggccttgatctttctcaggtagaactcca
actccttcccttccagtacataaccatcagctctaccacactgtcccggtctggaggctatgcatgcaagcagtttgccttgctggaactgttcttccaa
aagtgggcttatttttgctgtctttttcctctcctcatatttcttctgtgttttcttagatcgtttcttgtttaggatttcttcctcttcaggggtgagt
ttagctcccttaatgcgtcctagtgggagagcatagtgggcctcaaaccactgtctgtaaggagtgctgtcaaccaggacaatgcagttcttcacaaggg
ttttggttcgaaccagctcattgtttgacgcattgtacaccacatcaatgatcctagttttacgtgtgcaacactctgaaccccatgaaaagttgccagc
atccaacctcagagcacgatactttttgttaccaccgcggactctcacagtgtggatacggcgaggaccaatcttggtgtttgcagcaggacggccaagt
tcatactttctctttttgtgataaagcttccttttggcaccagtcttgcggcgcttgggccagttgtcccttgaaatacccattctcaagcgctggctgg
gaagaaanagcggggggggggggggaattaattaactccaatgcaaaaagaaaaattaacttgtattccggccccggaaaaaaccaaagtgcaattggaa
cctgggaatggacacctggggaaaaaaaggcg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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