XB-CLONE-4350338
Clone Name: IMAGE:7025862
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Gene:
ssr3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077002
[+]
ATTCTGACCCCGCCCATTTTCCAAGCGCTTCCGCTCCCTGAGTGCTGACGAATCAGTACAGAGATTTCTCTGACGGTGGCTGAGGAGGGTCATTCAGTGT
GAGACGTGTCTCTACTGTTCATTCTCTCTCCGATACCGGCCATGGCTCCGAAAGGTGGTAACAAGCAGCAGTCCGAGGAAGATCTGCTTTTGCAGGATTT
CAGCCGCAACCTGTCGGCTAAATCTTCAGCGCTTTTCTACGGCAATGCTTTCATCGTGTCTGCAATCCCCATTTGGCTGTATTGGAGAATATGGCACATG
GATCTGGTGCAGTCGGCTGTGCTATATACAGTAATGACGCTGGTCAGCACCTACCTTGTGGCTTTTGCATACAAGAATGTTAAATTTGTCCTCAAACACA
AGGTGGCGCAGAAACGCGAAGATGCTGTTTCTAGAGAAGTGACCCGAAAACTTTCGGAAGCAGACAATAGGAAGATGTCCAGGAAGGAAAAGGATGAAAG
GATCCTTTGGAAGAAAAATGAAGTCGCTGACTATGAGGCAACTACATTCTCCATTTTCTACAATAATACATTATTCCTGGTTTTGGTGATTGTTGCTTCT
TTCTTTCTTCTGAAGAACTTCAACCCCACGGTAAATTACATTTTATCTATAAGTGCTGCCTCTGGACTTATTGCTCTGCTTTCTACAGGATCAAAATAAA
TGGACAAGGTTATTTCAAAGACATTCATAAAAAAGGGTTGTTTTCTTTTTTTTAACAAACTATGTAAAATAAACATCTTTTTTGTTTGTAAAAAAAAAAA
AAAAA
5' EST: CF785588
[+]taattaatcccccccccccccattctgaccccgcctattttccaagcgcttccgctccctgagtgctgacgaatcagtacagagatttctctgacggtgg
ctgaggagggtcattcagtgtgagacgtgtctctactgttcattctctctccgataccggccatggctccgaaaggtggtaacaagcagcagtccgagga
agatctgcttttgcaggatttcagccgcaacctgtcggctaaatcttcagcgcttttctacggcaatgctttcttcctgtctgcaatctccatttggctg
tattggagaatatggcccacggatctggtgcactcggttgtgctaaataagtaacgacgctggtcagcacctaccttgtggttttggcatgcaaggatgt
taaaaattttgtcccccaacactagagtggccgccggaaactcttaagatgctgggtttttagaaaaagtgagccccgaaatcttttttgttaagccctt
acaacccgttttgtacgccccccagtgtaagagaatacaacatttataaaggaactcctcttttgacaagacaccaaaacggcagcggtccgtgtctgaa
atattatattaaagggctacccggtttacaagtataattttcgcaacgtcgcgtcttggtatatctaaacgcccggccgcgcggcgttttttttaattat
atcaccttggggagggcgtcgccaactgcgtgctctggctatttttctgcgatgttatcagcgcatatccgcttatttttttgggtggttgtgaacgcgc
gacagtcttgatgatagggttaaaggctctgtgtgccaaaatttcacactctttttttagtcggccgcctcgacacaacactcaaacctttatcgtttct
ttggttacgccgctttattgtgaaagatggggtgatcgcataaccatgccatcaccggagatgcgaaccgtagaaataagaaagaaaaataacacacgcg
gctatttaaagggtgatattgaaatggtcattttagagggtacagancnangaattatgggagatatcactaagccgcgaagcgattatagttttcctgc
aggttagcctctcttcctgcgttctgtgttggtcacggtggtctcgtggaattatatatcgtttgcgttggggttgttcgggacgctcaccaccacgccc
tctaaaattaggttaattaattctcgctcgactccagtat
3' EST: CF785589
[+]tttttttttttttttacaaacaaaaaagatgtttattttacatagtttgttaaaaaaaagaaaacaacccttttttatgaatgtctttgaaataaccttg
tccatttattttgatcctgtagaaagcagagcaataagtccagaggcagcacttatagataaaatgtaatttaccgtggggttgaagttcttcagaagaa
anngaaagan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: