XB-CLONE-4350335
Clone Name: IMAGE:7025861
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Gene:
atp5mc1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077049 [+]
GCTTTTTTCCTGTCAAAGCGTCTGCACTGCGCATGCGTAATTCCTTCGCTCGCCTTCTGTCTTTCCCCGCCAAGCAGCGAGTGCTATTCTTGTTCCCGCG
TCTTGTTCACTTTCACCCCAAATATGTACGCCTGCGCCAAGCTTGTGTCTAACCCAGCCCTGGTGAGAAGTGGAGTCTGTCTTCTCTCTCGACCAGTTTC
TGTCCCACTACTGAGCTACACTGGACTGAGGACTGAGCAGCTGATGCCTGTTCCAGCTAGGGGAATCCAAAGCAGTGTAACATGCAGGGACATTGATACA
GCTGCAAAGTTTATTGGAGCTGGTGCTGCTACCGTAGGTGTGGCTGGCTCTGGTGCTGGTATTGGGACAGTGTTTGGCAGCCTTATCATTGGATACGCAA
GGAATCCTTCTCTGAAACAACAACTCTTCTCTTATGCCATCCTGGGGTTTGCCCTGTCTGAAGCTATGGGACTGTTCTGTCTTATGGTCGCTTTCCTCAT
CCTGTTTGCCATGTAAAACATTAAGATCACCAGATTATATTTTGGACCCCCTGTACTAGGGCTTTGTGGTGAGGGTAGATCCCTCTTCACTCTGCTGCTG
TATAATCTGATGTTCCTGGGGAATAAATATTATAAATACATTGTGGGTCTCATATTTCAGGAGCTCACTCTAAAAAAGTAGATGTGTGCATAAAATTCTT
TGAATGGACAGATGGTATTATTAAAGTGGTCACCTGGACAATTCTTCTACTGTCAAAAAAAAAAAAAAAA
5' EST: CF785591 [+]taattaatcccccccccccccgcttttttcctgtcaaagcgtctgcactgcgcatgcgtaattccttcgctcgccttctgtctttccccgccaagcagcg
agtgctattcttgttcccgcgtcttgttcactttcaccccaaatatgtacgcctgcgccaagcttgtgtctaacccagccctggtgagaagtggagtctg
tcttctctctcgaccagtttctgtcccactactgagctacactggactgaggactgagcagctgatgcctgttccagctaggggaatccaaagcagtgta
acatgcagggacattgatacagctgcaaagtttattggagctggtgctgctaccgtaggtgtggctggctctggtgctggtattgggacagtgtttggca
gccttatcattggatacgcaaggaatccttctctgaaacaacaactcttctcttatgccatcctggggtttgccctgtctgaagctatgggactgttctg
tcttatggtcgctttcctcatcctgtttgccatgtaaaacattaagatcaccagattatattttggaccccctgtactagggctttgtggtgagggtaga
tccctcttcactctgctgctggtataatctgatgttcctgggggaataaatattataaatacattggtgggtctcaaattttcaggagctcactctaaaa
aaataaaatgtggggcataaaaatttcttttaaaggggaacaaatgggtattatttaaaagtgggtcaacctgggaaaaatttccttcttactggtcccc
aaaagaacaacgcggactaaagtata
3' EST: CF785590 [+]tttttttttttttttgacagtagaagaattgtccaggtgaccactttaataataccatctgtccattcaaagaattttatgcacacatctacttttttag
agtgagctcctgaaatatgagacccacaatgtatttataatatttattccccaggaacatcagattatacagcagcagagtgaagagggatctaccctca
ccacaaagccctagtacagggggtccaaaatataatctggtgatcttaatgttttacatggcaaacaggatgaggaaagcgaccataagacagaacagtc
ccatagcttcagacagggcaaaccccaggatggcataagagaagagttgttgtttcagagaaggattccttgcgtatccaatgataaggctgccaaacac
tgtcccaataccagcaccagagccagccacacctacggtagcagcaccagctccaataaactttgcagctgtatcaatgtccctgcatgttacactgctt
tggattcccctagctggaacaggcatcagctgctcagtcctcaatccagtgtacctcagtagtgggaacaaaacgggtcgagaaagaaaacagactcccc
tttctccccagggtggggttagaccacaagcttggcgcaaggcgtcatattttgggggggaaagtgaaacaagaagccgggaacaagaaataccacctcg
cttccctgggcggggaaaagaaagaaagggcgagcgaaagggatttaccccctggccccattggcaaacaccttttgaaagagataaaaaaagcgggggg
gggggggggggaatatatatcaccctcgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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