XB-CLONE-4350330
Clone Name: IMAGE:7025859
|
Gene:
smdt1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF785594
[+]
taattaatcccccccccacccgaggcgtcttgtcttcccaaacatggcggctagagtcggtgttctctctgttgcagggtttagagcggcagcgagagca
ggggggctcttgagagcatcaaagcagtcgtcagctgttagccatgtaccctgtcggacggcaatagccaccagcgctgggacagttcttccaaagccgg
aaaaagttagctttggccttcttcgagtgtttactgtggtcatcccattcctgtacataggaacccttattagcaagaactttgcagccctgctggagga
gcatgatatctttgttccagaggatgacgatgatgacgattaacaactttcttcatacggtgttcacaaggagccttatcacgtgaagatggacctttgt
ctgcagcaatgggcagaactaaagttgccggaaccaaatctacacagaagctaaactgataatttgctgcatgtttataaaaggaaactgatattttgtt
gttttgcctccaccatttgaagggaggttgttgcctgggttccaaggtgaaagaagcatgtggtgttatagggtaatagtaataaatatctgttacaaaa
ctgtaaaaaaaaaaaaaggatccccttagttctaagagcggccgcccaccgcgggtggaaccttgggccgttgggcccaacttggttttatttgcgagct
ttaataatagtttttaccaaattaaaagccaataagccattcaccaaaaattttccacaaaaaaaaaaaggcatttttttttttctcaccgtggcgaatt
tcttaaaatttgtgtggggttnttgtggttccaaaaaccttccaagaggaaaaggggttattcct
3' EST: CF785593
[+]tttttttttttttacagtttgtaacagatatttattactattaccctataacaccacatgcttctttcaccttggaacccaggcaacaacctcccttcaa
atggtggaggcaaaacaacaaaatatcagtttccttttataaacatgcagcaaattatcagtttagcttctgtgtagatttggttccggcaactttagtt
ctgcccattgctgcagacaaaggtccatcttcacgtgataaggctccttgtgaacaccgtatgaagaaagttgttaatcgtcatcatcgtcatcctctgg
aacaaagatatcatgctcctccagcagggctgcaaagttcttgctaataagggttcctatgtacaggaatgggatgaccacagtaaacactcgaagaagg
ccaaagctaactttttccggctttggaagaactgtcccagcgctggtggctattgccgtccgacagggtacatggctaacagctgacgactgctttgatg
ctctcaagagcccccctgctctcgctggccgctctaaaccctgcaacagagagaacacccgactcctaaccccccatgtttgggaaaaacaaaaacgcct
tcgggggggggggggggattaaattaactcgaggtgcaaaaagaaaaaagtaacctggtatttcggggcccgggaaaaaaaccggagggggcactttggg
aaccgggggaaatgggaaaccctgggggaaaaaaaaaaggccaaaagggggaattttattttcctaataaggggct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: