XB-CLONE-4350325
Clone Name: IMAGE:7025857
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Gene:
trg
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785597
[+]
taattaatccccccccccccccccccgagcacgaagggacagagtacggagcaggaaacagcgtgttatgcaatttgaaacttgagacacggtgaatgta
caccatttactttgagccaaccatatatttctatcttcaggaatgcctcacaggacaaatcggtcagtgttaaatgtaactggaaagatattaatctgca
aacacaagacatatattggtatttgcagagacccaatggagaagtaaaaagaattttacatgctataagagaaactcaagaatttgacaatggatttagt
gacatcaagtttgatcttgactttgaaaaaaccttaacaatcaaaagtattgtggaggatgactctggaacttattattgcgctgctttgggaggacacc
gcataaaaatctttgggtctggcacaaaactcattgttggccatactgcacggtcagctgaacaacctaaatctactatactaagcccatccttgaaaga
aatggagagaaacaggccaaggaatatacctctgcctgcttgagaacttctttcctgatgtaatccaggttgtttggaaaagtggaaaccaagatattcc
atcagaacaacaagaaataaaaggggttccagaaaaagatggcaaataccttgtaattcagttaccaagctggaattaaggttatcttaaaattgatata
aggccaagagggttcaccctggccgcttattaagcattgtaaggggtttaatgccaaaccttggagcaacctgggaaagaaaaatatcttggtaagccgg
aaaaaaccaaggcccaccctataaaagttggtccccctgggga
3' EST: CF785596
[+]tttttttttttttttgctcagaaatctttatttttagaaaataaacatgccaggaagcttgttaacatatattgtagtacaggcatggaatcccttaccc
ggaaaccatttatacagatagctctgaattacgccatctcccatagactcaattttaaccaaataatttcaatttttaaaaacaatatccctttttctct
gtaataattaaacagtagcttgtacttgatggtaaccaaattgtatgaatccatattggaagctaaaaaatcttactgggtttatttaatgtttaaatca
tttttaagcagacttaaaggagcagttccataagtctttccataatctggatctatataccttaaaggaacagttcagtctaaatgggtataaaagacag
actgcgcaaaataaaaaatatttctaatagttaggcaaaaattcaatctttaaaggctggagtgtgcagtgtctaacatagtagccagaacatagtactt
cctcctttacagttgtctaagctgttagtggtcagtagccaatcagtgacttgggagggcacatgggacataaccgttgagttaatttgcatttaaatct
gacctgcgtgctcacaaactaactgaaaaattatgtcccatgggcccccctaaagtcactaagaggttaaagagctgaaagcaggaagtagtgtgctggc
cattatgttaggcttctgtccgctccagccttttttacctctattttacccagtttcatttttaactgaactgtttctttaaaggatagtggatcaaaat
atgggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: