XB-CLONE-4350317
Clone Name: IMAGE:7025854
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Gene:
tuba1c
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785601 [+]
taattaatccccccccccccccaaggcagttcttaccgagactccttgctgtcggtcggtgagaagccgcaagctgtgctttcccattccgtaaaactat
ctgctgtctaacagaccccatccgagtcagtcatgcgtgagtgcatctctatacacgttggccaggctggtgtgcagatcggcaatgcctgctgggaact
gtattgtttggaacatggcatccaacctgatgggcagatgcccagtgacaagaccattggaggaggagacgactccttcaacacctttttcagtgaaact
ggagcaggaaagcatgtccccagggctgtctttgtagacttggaacccactgtcattgatgaggtgcgcactggcacatataggcagttattccacccag
agcagttaattacaggaaaggaagatgctgccaataattatgcccgggggcactacacaattggcaaggagatcattgatctggtgctagacaggattcg
caaactggctgaccagtgcacaggtctccagggtttcctggtcttccacagctttggtggtggtactggttctggattcacctccctgctgatggagcgt
ctctctgttgattatggaaagaagtctaagctagaatttgccatctatccagctcctcaggtctccacagctgtggttgagccctacaactctattctca
caacccacaccacccctgaaacactccgactgtgcttttgtgggaaaacaatgaaggccatttaggact
3' EST: CF785602 [+]tttttttttttttttcacatttaaaatggcgctttattttaaaaaaaaaagttagacttcagaatgggaaattcaggaatagaaaggcacaacttgagga
aaacagaataaagtttttaatactcttcgccttcatcttctgcatctgcactatctgcaccaacctcttcataatctttctccagggcagccatgtcttc
acgggcctcagagaactcaccctcctccataccctcacccacataccagtgcacgaaggcacgcttggcatacatgagatcaaacttgtggtccagacga
gcccaggcctcagcaatcgcagtggtattgctcagcatacacacagcacgctgcaccttggccaggtctccccctggaaccacagttgggggctgatagt
tgataccaactttgaaaccagttgggcaccaatccacaaactggatggtacgctttgtcttgatggtggcaatagctgcatttacatctttgggtaccac
atcaccacggtacaacaggcaacaagccatgtatttaccgtggcgtggatcacatttcaccatctggttagctggttcaaagcaggcatttgtaatgtca
gctacagtaagctgctcatgataagccttctctgcagagatgacaggggcataggtggcaagagggaagtgaatacggggatagggcaccaagttggtct
gaaactctgtgagatctacattgagggctccatcaaacctcagagaagctgtgatagaagacactatctgactaatgagcctgttcaggttgngtgtaag
n
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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