XB-CLONE-4350311
Clone Name: IMAGE:7025852
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Gene:
tax1bp3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080496 [+]
CTGTGAGCTAGCAGCCATGTCTTACATCCCGGGCCAGCCGGTCACTGCTGTAGTGCAACGGGTTGAAATCTATAAATTGCACCAAGGGGATAATCTGATC
CTTGGCTTCAGCATTGGAGGAGGAATCGACCAGGACCCGGCACAAAACCCTTTCTCCGAGGACAAGACAGACAAGGGTATCTATGTGACACGAGTAACAG
AGGGAGGCCCTGCGGAGGTGGCTGGACTTCAAATTGGAGACAAAATAATGCAGGTAAATGGATGGGACATGACAATGGTAACGCACGACCAGGCAAGGAA
ACGCCTCACCAAGAAAAATGAGGAGGTTGTCCGGCTGCTTGTGACCAGAAAATCCTTGCAAGAGGCTGTGAGACAGTCCATGAGACCCTAAGCATCACAT
TCCCGAGAGTCTGAATCTCTTAGCTACACAGGTGTACTTACCCGGACACATTTCAGTTCTGTATTACACACACCAGCAGCACATTGCAGGGCTATAGGTT
GCTGGCTCCTTTCTGTCCCTCCCAGGCTACTGCTTGTTCCGCACCAAAACAAACCAAAAAGAATTCTAATGTGCCTGGCTCCCATTGGCCTAAATTCTGC
CCGTCTTTATCCTTGCAAATGAAAGAGCAATTTCTTACTGCCCCGGTAGAAAAGCACACATCTTTCCACTGCAAAGATAAAGCAGGTAGGTATCTCCTAT
GCCCTAGTATTGAAATAGGGAAGATCACCAGGTGGCCTCTCCAATGCGATTCCCAGTTCACTTTTTTAATACTTTCAGGTGTTAAGTTGCTTCTATTGGA
GCAACCACAAATATGGGGACTTTATTTTACAAAATTCAACATGTAAACAGAGGTCCCTGTATCACTGGTTTATACACAGATTTGTACTTGCAGAGCAAAT
AGTCTCTTCTAGGAGGGGAAGTAGAGTTTGTACTGAGTCATCCTCGCCTTTCAGCATTTATCACAAGGGCTGCCATCAGCAGTCTCTGGGTACAGCCTGG
GTCACTGCCTCTAATTACCGTCAGGGTCAATCAGCTGAATCAATAAATGCTTAATCTAGGCAGAATTTGTAGGTGTAAGCGCTCTTATCAATTTGACTTT
TTTCATGATATTACCAGTTACTACACTGCTGATGTGAATGTGTAACAGGAGTGCCCCCTGTTTTTGGGCCCAGGTAAGGGCCAGACGAATGGCAAAAGAT
TAATTTACAGAAGCAGAAAGCCTCCTGTCGCTTCTCTGCTCAGTTTTATTCTTACCCTCTTGGCACATTACAAGAGACAACTGGACAAGAACCCTACCCT
GAGCCCAATCAACAGGGGACACTCTGATATTAGCAGTGTTAAAACTGCCAATGTCGTCAAATCTAAAACAAAATTTACCTATAAATTGCAAAAGTGCTCA
GAAGAGCATTCTGGACAATTTTACATTGTTCCATTTAGGGTATGTGTCTCTTTGAGGGGCTTCAAGACCTAGTGCTGTCAGAGGTTACTGACTTTGGCGC
CCAACTGGCCAAGGTTTAATAGGAGAATCCAGGAAATAGATTGCCCAGGGAATATACTGCTTTGTTACTGGTGTTACTACAGCACAGACAATCAGACAAG
TTCCCCTGTCCCCTTTTATCTATATCTCTAGCAATTCATATGCAGTCCCACTGCCACCAGCAACTGACTGGCCTGTACTAATTTATATAAATATATATAT
ATGAAACCACTCCATATAGCTAGTTTAACTGTCTTGTATCATTTCCAGTTTTAACTGTACTGTATGTACTTTGCATTACTTTTCTAAATTTCTGACCAAA
CGATATTCCTAGGCTTGTAATAAACTGCTTTTGATTGTGGCAAAAAAAAAAAAAAAA
5' EST: CF785605 [+]taattaatcccccccccccccctgtgagctagcagccatgtcttacatcccgggccagccggtcactgctgtagtgcaacgggttgaaatctataaattg
caccaaggggataatctgatccttggcttcagcattggaggaggaatcgaccaggacccggcacaaaaccctttctccgaggacaagacagacaagggta
tctatgtgacacgagtaacagagggaggccctgcggagggtggctggacttccaaatgggagacaaaaaaaatgcaaggtaaaatgggatggggaacttg
gccaattgggtaacccccccaaccccggccaagggaaaaaccccccccccccagaaaaaaattggggaggggttttccccggcttccttttttaaccaaa
aaaaaaccccttttaaaaaaggggtttggaaaaaaccccccttgaaaaccccccttaaaggccttctttttttttccccaaaaaaaggtggaaaatctct
cttttaaaccacacaaggggggggggtttttttttccccgggggaaaaaaaattttttttagtgtttttggtggggaataaaacaccccccccccccncc
gagggcgcccaaattttttttttttggggggggggggggaaaaaaaaaaggtgtggggggggggggccccccccttttttttttttgttttgcccccccc
cccccacaaaaaa
3' EST: CF785606 [+]tttttttttttttttgccacaatcaaaagcagtttattacaagcctaggaatatcgtttggtcagaaatttagaaaagtaatgcaaagtacatacagtac
agttaaaactggaaatgatacaagacagttaaactagctatatggagtggtttcatatatatatatttatataaattagtacaggccagtcagttgctgg
tggcagtgggactgcatatgaattgctagagatatagataaaaggggacaggggaacttgtctgattgtctgtgctgtagtaacaccagtaacaaagcag
tatattccctgggcaatctatttcctggattctcctattaaaccttggccagttgggcgccaaagtcagtaacctctgacagcactaggtcttgaagccc
ctcaaagagacacataccctaaatggaacaatgtaaaattgtccagaatgctcttctgagcacttttgcaatttataggtaaattttgttttagatttga
cgacattggcagttttaacactgctaatatcagagtgtcccctgttgattgggctcagggtagggttcttgtccagttgtctcttgtaatgtgccaagag
ggtaagaataaaactgagcagagaagcgacaggaggctttctgcttctgtaaattaatcttttgccattcgtctggcccttaccctgggccaaaaacagg
gggcactcctgttacacattcacatcaacagtgtagtaactggtaatatcatgaaaaaagtcaaattgataagagcgcttacaccttacaattctgccta
gaataagcattttat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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