XB-CLONE-4350302
Clone Name: IMAGE:7025849
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Gene:
anxa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785612
[+]
taattaatcccccccccccccgagcctgctgcttccctgtaacatctactagtgactctttcttgtcctcctccagaaactaccgtcatcatgtctttca
tgcatgaattccttactcatgcatccttcctggaaggtggaggacaccccccatgcactgctgaagaatctggcccatcacttaaagcaaatcctggatt
caatgctgcagctgatgcagcaaacttggataaagccattaaagcaaaaggtgtggatgaaggaactatcattgacattctgacaaaaagaaccaattgt
gaacgacagcaaatcagagctgcttatcaacaattaaccgggaagagtcttgatgatgctttgaagaagtgcctaaaaagtcacctggaggaagttgtcc
tgggtttactcaaaactccagcccagtttgatgcccatgaattgaggggtgccatcaagggccttggaacagatgaagactgtttgattgagatcttggt
atcgaggaccaattgtgaaatcaaagaaattaacaaggtttataaagaagaatttaaaaaggaacttggaaaggacattcttggtgacacatcaggagat
tttcagaagactctccttgccctctctaagggggaacggaatgaggatacacgtgtcaatgaagaccaagctgacnacgatgccagggcattgtatgaag
ctggnagagaagaaaaaaggaactgacgtatctacattcatcaacattttaaactaataaaagttatccacatatttcagaaagttttacaagagatatg
tcaaggatacagga
3' EST: CF785611
[+]tttttttttttttttttttttttttttttttttttttttgcacaaaatgttttgttttattaaaaaattacaaaaatgtttgcagtataatgtaccaaaa
aaaacaggtctgtaaagggatgcagtttgcaatgtaaatggacaactgtagtaactttattttaatatttccaccctgaggacaagttaaaccaagcaaa
gctcccatataacaattatagaccaatggaatttatacagagtttcaggagtcagggccacacagggccaacataatggtttcataatcccctttgaatt
tttcttccataataacccggcggaaaaatttgccataaagtcctttataacagggtttgatttcctttaaggcaatttcagatctggaaactagtactct
gaataaagcattgtgcccagttccaaatcccttcatggccaaataaaatctttcagcaaaataaagcgggtttagaactagcgcatttcactatactcct
taaagcattttttcccgatctcctttcctctcaagatcaaaggccccggttcaaatcattttttgccggaacctgcctatcctcgggaaaaaatttctga
aaaatgtgggaaaacctttttaataagttaaaaaaggtttaaaaaaaagttaaaataccttcagtttccccttttccccttcccccccagcttttaaaaa
aaaagcccccggggaaacctttgtccaacccttgggcccttccatgggacacccgggtaaaccccccaatttcccgttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: