XB-CLONE-4350289
Clone Name: IMAGE:7025844
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Gene:
sumo1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077048 [+]
GACACCTTCCTTGTCTGGTTCTCTTGTGCTGTTATTGTGCCCGGAGGAAGGAAAAGCGTAGAGCGGTCCAGTGTAGCGAACCGATACCGGCCGAGAGCAG
TTAGCATGTCTGATCAGGAAGCTAAACCATCTAGCGAGGATCTAGGAGACAAAAAAGAAGGAGGGGATTATATCAAACTCAAAGTCATCGGACAGGATAG
CAGTGAAATTCACTTTAAGGTAAAGATGACAACGCATCTCAAAAAGCTGAAAGAGTCATACTGTCAGAGACAGGGTGTTCCAATGAATTCTCTCAGGTTT
TTGTTTGAAGGGCAAAGAATCTCAGATCACCAGACTCCTAAGGAGCTCGGTATGGAGGAAGAGGATGTTATTGAAGTTTATCAGGAACAGACTGGGGGCC
ACTCGACAATTTAAATTTTTCCCTCACCTTCGTTTTTTTTTTTTTTTTAATTATTATTTTTTTTACTTTTTTTTTTTTTTTGTAATGTTTACCTCTCCTT
CCCCTTGGTACTCTGTTTCCTTTCATGTAATCAGCCAGATGTCTGTGGCATCTAGATCACCTCATTTTAATATCTTCATCAGTATGAGAAGTCACTTCTG
AAGATGTCACCAGTCCAGGCTGTTTTAGCTTCACTCCTTTTGGTCTCCAAGAATATTGATTTGAATTTACATTGTAAAATTACTCGGTTGCTTGGGACAC
GAAAAAGAACGTTTTTTGGGGATTTATTGCACTTCAAATTGTTGAATAAAATTTTCACCATTACTGTTATCGTTCCACCTGCCTAGAAGATATAGGAGTT
GTGTTTTTAGATACAAGTTGTCATGCAACCACCCCCTATGAAATGTTCGCTGTATCTTTTATTTTTGTGGGGCATGGGTTTTAAAAAAAAGTAGAAAAAA
AAAATAAAAAAAAAAAAAAAA
5' EST: CF785619 [+]taattaatcccccccccccccgacaccttccttgtctggttctcttgtgctgttattgtgcccggaggaaggaaaagcgtagagcggtccagtgtagcga
accgataccggccgagagcagttagcatgtctgatcaggaagctaaaccatctagcgaggatctaggagacaaaaaagaaggaggggattatatcaaact
caaagtcatcggacaggatagcagtgaaattcactttaaggtaaagatgacaacgcatctcaaaaagctgaaagagtcatactgtcagagacagggtgtt
ccaatgaattctctcaggtttttgtttgaagggcaaagaatctcagatcaccagactcctaaggagctcggtatggaggaagaggatgttattgaagttt
atcaggaacagactgggggccactcgacaatttaaatttttccctcaccttcgttttttttttttttttaattattattttttttacttttttttttttt
ttgtaatgtttacctctccttccccttggtactctgtttcctttcatgtaatcagccagatgtctgtggcatctagatcacctcattttaatatcttcat
cagtatgagaagtcacttctgaagatgtcaccagtccaggctgttttagcttcactccttttgggctccaagaatattgatttgaatttacattggtaaa
attactcgggttgcttggggacacgaaaaaaaaacgttttttgggggatttattgcacttcaaattg
3' EST: CF785620 [+]tttttttttttttttattttttttttctacttttttttaaaacccatgccccacaaaaataaaagatacagcgaacatttcatagggggtggttgcatga
caacttgtatctaaaaacacaactcctatatcttctaggcaggtggaacgataacagtaatggtgaaaattttattcaacaatttgaagtgcaataaatc
cccaaaaaacgttctttttcgtgtcccaagcaaccgagtaattttacaatgtaaattcaaatcaatattcttggagaccaaaaggagtgaagctaaaaca
gcctggactggtgacatcttcagaagtgacttctcatactgatgaagatattaaaatgaggtgatctagatgccacagacatctggctgattacatgaaa
ggaaacagagtaccaaggggaaggagaggtaaacattacaaaaaaaaaaaaaaagtaaaaaaaataataattaaaaaaaaaaaaaaaacgaaggtgaggg
aaaaatttaaattgtcgagtggcccccagtctgttcctgataaacttcaataacatcctcttcctccataccgagctccttaggagtctggtgatctgag
attctttgcccttcaaacaaaaacctgagagaattcattggaacaccctgtctctgacagtatgactctttcagctttttgagatgcgttgtcatcttta
ccttaaagtgaattcactgctatcctgtccaatgactttgaatttgaaataaccccctccttcttttttggcccccaaaatcctccgctaaatggtttaa
actttcctgatcaaaaatgctaaacggcta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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