XB-CLONE-4350258
Clone Name: IMAGE:7025832
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785638 [+]
taattaatccccccccccccctggcacttcgcattcggcttccaacttgtgttgtatcgtccgaagatcatagaacgtctcaccatgtcttgtgatgaag
gaaaactctttgtcggtggtctgaattttgaaaccacagaggaaagcttggagcaagtgtttagcaaatatggacagcgttgctgaagtggttgtggtga
aggatcgggaaagcaagtgatccagaggctttggctctgtcacattcgagaattccgaagattccaaggatgctatgatggctaatgaatggtaagcctg
tagatggctttcacattcgtgtttatcccgctggtaattcctccatcgtaatgagacgtggtttctgggggggccgcgcctgcggccagcgggttcacca
aaccttggtaccttcccattctgtagccgtgtgcctagttcccgttccgcgttttatccctctgccatcctgcctcgttttttttccaggtttttttgca
cagggtccgggagttttaccaattttatcatttcccttccgtggtcgtctgtcgttgtgttcaaccccccgtctagatcgatatcgttatcaaatccaca
aggggccgaatcaaccgtagttttttcgtccccgcgtacatttaacctatggagcgttgtgttacccgaaacgagtgatcagattcaatctatatccgcg
tctctgttctctctcttccatatttttccgcgtgggcggcggttatagcgggtgtgccgcgatttatgtattcaccccctccccccctctctattattgg
cttatcgccgagggagtttaaaccatgagtggggttggccgcgcccgattttattttacaatataaacaagtaacacaaatgccgccttcggtgtaggtg
cataagggggggtcgcgctgtttttcgcacgtatggtgagtacatttgtttttcgtagaaagcatgtggttgtgttttggacctacatttggcgtgttcg
gcgcttttttgagactctttgtcccccttagtgagtttaccaagcgatctgttttcgcttatccgcgctcatgccactataatagcattccggttcttta
tttatccctcattagttagagctctcggttctgggcgtttttacctgctcgcccctctcgctcgttg
3' EST: CF785639 [+]ctgctttggtaacctccacttctgttttcaaagcggctgcttccaccatatcctctgtctccaccaccacctcggcctctgcctccacggaagaaccctc
tgcctccagaagagccacctctgtaaccacctctcctatcattggaggacttgccagcctgatcaacacggatttgacggccatctacagacttcccatt
cattgccatcatagcatccttggcatcttcaggattctcaaatgtgacaaagccaaagcctctggatctcttgctttcccgatccttcaccacaaccact
ttcagcaacctgtccatatttggctaaacactttgctccaaagctttccctctgtggggtttcaaaaaattcagaccacccgacaaaagaattttttcct
ttcatccaaagaacattgggtgagaacgtttccatgaaatcttcggacgaattccaaccccccttttggaaaaccccaaaacgccaaattggcccaan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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