XB-CLONE-4350252
Clone Name: IMAGE:7025830
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Gene:
dhx35
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785642 [+]
taattaatcccccccccccccgagggtaaaatgagggcatttcacagcgaggacggatatttttcttggaaaagctgtgtaattagtgattgtacaggaa
tcaggaagaaaagacaaatggcataggcaggaggaacgatagcgggacagctgactggagggacagaatgtgtgcattttttcatgttgaatcgagaaaa
cgctacagctgaagtagaagcaagctgccttggaagctgtcgctaagttatcgtctaccacttacagtagcgactaccaaccaatcacacgtttcgcttc
tgcgcatgcgtcctgtatcatactgcgcttcctgagccatcaacaaatatggctgcgcccgtggggtcggtacggtcctggacaccgggcattgatagtc
tatgcctacaaccatttctgcataagaatgcaaaataaaacttcagagtcccctcccgctaatttaccctcccccttcgccctcctttttttccccctat
ttgtgaaaaggtccgcataccaatgcctattttttttttacgttcgagcgccaaatctatattataaggaccgcgctatagtgggtaaggaatttttcct
tttcgaatacgcgcggaagatacgcatacttgattctgcgcgcgcgctggcacatctatcttgtccctcggctctggcggttcgccaaataaactacttc
tatctacctctatgaattaattaaatccgtcctgtgttttcgaccatgctttccaccatctctttttaccccctaatctcccccccgccgctatttgtgt
ccgctcgtttctggctccaccctataaattttattataagaaggagtctcccccgctcaaaaatattggttacacgcccctttattccccgccatcaaga
ttttttacggaggggtgtggcgggtttttaatcccccccgggcgggtatttctcttgacacgaactccgtagggtaggtgctccacttgagacccccgtt
tccggtgatgaatgagggtntctcatagggaattacccccgcctattatcatccgtgtgtttttgtgcagtctcattttggtggattgcagagaccaaag
cttcagttggagtttttttatgggatgggggcgcttatggtgggtttttccccccttattttatcatacaaatctttatgtcgcgtgaattgcgctatat
ttcaccccattttattctttttacgtttatcccgcgcgcggtgcgccgtttaatatgggttctccggagtgatacacactaccctgaataaggtcacgct
tcgcctgttctctccgggcttaaacaccgcgccgcttctcacagatagg
3' EST: CF785643 [+]acatcatttctctaacaagcatcccatctgtcaaaaacttgatacgtgtggcttgtggatcagtacagtcgtcaaagcgtatgcaatagccaacctcatg
ccccagcacagctccacgctcttccgccacacgtccggctaccgaaactgctgctaccctacgtggctgggtaacccccactactcgtccttctgctgtc
catccggcctctgtcaggtactgcggtatctgggtgcttttcccacaccctgtctctccaataatcaccacagtctggtaattctccactaggtacagga
tgtgatttctcagcttaaacacaggcagcttctgtctctgatgctctatggagagagaggcatgtgggttgtagatgacagaggagacctcaaaattact
ctgcctctcttcagcaatggtggcgcctgggccttcagtgcccggcttccagaaccgtaccggccccacgggcgccgccatctttgttgatggctcagga
agcgcagaatggtgcaggacgcatgcgcagaagcgaaacgtgtgattggttggtagtcgctactgtaagtggtaggcggtaacttagcgacagcttccaa
ggcagcttgcttctacttcagctgtagcgttttctcgattcaacatgaaaaaatgcacacattctgtccctccagtcagctgtcccgctatcgttcctcc
tgcctatgccatttgtcttttcttcccgattcccgtacaatcactaattacacagctttttcaagaaaaatatcccgtcctcgctgtc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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