XB-CLONE-4350246
Clone Name: IMAGE:7025828
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785646 [+]
taattaatcccccccccccccatcaaatgtataagaacgcaacttgttgcatatgaaaaaatgcaatgacacgactgcagatttgactgatttgctcgtt
caggtgctcttaaaactacatgtcaaacacacaaaaataataatgcctgtgtataagggccctaatgtaaataggattctacaaaaaaagaaaaaaaaaa
tatgaatgtgccttatattctctagaatcaaagtatctttccatgtttctataaggcgccattttttatttaaaggagcagttcaccttaaaattaactt
tttggcatgatgtaagtctgttctacagactttaagcttaaaatttcaactgccgactggttactaagggtaattaccccagcaacctggcagtaggtga
cttgaagttcaatagaacgatatgcaataaaactgaaactacacattcaaattgtttgtttctaaccttgacaccaatttaacttttttaattgcatgct
ggaaacaggtggaaaggaaaggctacctttttagcgactatgctaattacattataaaaaaccattctaaaatttgcttaccaccaccccaattttttaa
gcttatttaaaaaaagtttcattcacaagagtgaaattttcaccgtctaaatatacaaccccctgtcttttacaataaaactatggttattgccaataat
ttttgccaccacctacttatacaatagacaatggctccgagagacgacaaaaaaatagtttatataacaaccacgcacccccccctcattcgtgcaccac
acggtctctataattatatatttcacaattttgtaaagatgagaaaaagagatcttgaattctatactgcttaccacacaacaacttcatta
3' EST: CF785647 [+]tttttttttttttttgcaaaaccatttttcttttatttcttacaattgtgcagtgattttcacttgaatgataatagatgtgagcatagtaccataagca
aatagtttttctaacaatacaaaaaaacaaaaacatttcatatagaaaatcctccaaatgatggcattacctgctttacataaaaccagaaactgcagct
taaaggggaagttcacctttgaataaaccttttttaattaagttatagatgcacctattctaagcaacttttcaattagtctttattatttagtttgcat
ggtccctaacataatagccttcctatccacctatttccagcctgcaactaaaaaagctaaattggtgtcaaggtcagaaacaaacaaattaaatgtgaag
tttcagttttattgcatatcgttctattgaacttcaagtcacctactgccaggttgctggggtaattacccttagtaaccagtcggcagttgaaattcta
agcttaaagtctgtagaacagacttacatcatgccaaaaagttaattttaaggtgaactgctcctttaaataaaaaatggcgccttatagaaacatggaa
agatactttgattctagagaatataaggcacattcatattttttttttctttttttgtagaatcctatttacattanggcccttatacacaggcattatt
atttttgtgtgtttgacatgtagttttaagagcacctgaacgagcaaatcagtcaaatctgcagtcgtgtcattgcattttttcatatgcaacaagttgc
gttcttaacattatgn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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