XB-CLONE-4350243
Clone Name: IMAGE:7025827
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Gene:
prmt1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785649
[+]
taattaatcccccccccccccccggcagttctctcgtacaaagccggtggcgtggctttgagtttcttttttagattcgtgagagagactgacaaacatg
gctgaagcgagcacctgcaacatggaggtttcttgtactcagcctgagagcagtgtgaagccaaatgcggaggacatgacatcaaaagattactactttg
actcgtatgtttactttggcatccatgaggagatgttaaaagatgaagttcgaacgctgacatatcgtaactcaatgtttcacaacagacatctatttaa
agataaagtagtcttggatgttgggagtggcacgggtattctctgcatgtttgcagcaaaggctggtgccaagaaagttattgggattgagtgctcaagt
atatctgactatgccataaaaatcgtgaaggcaaataaactagatcatgtggttacaatcatcaaaggcaaagtagaagaggtggagctcccagtagaaa
aagtggacatcataatcagtgaatggatgggatattgtctgttctatgaatcaatgctgaacactgttatctatgcacgggataaatggctgactccaga
tgggttaatatttccagatcgtgctacactgtatgtaactgctattgaggatagacaatacaaagactacaaaattcactggtggggaaaacgtttatgg
gctttgatatgtcatgcatcaaggatgtagccctcaaggagcctctggttgatgttgnttgatcccaaacaactggtcactaaggcttgcccttataaag
gaaagttgatattttatacg
3' EST: CF785648
[+]tttttttttttttttatttttttttttttttttttacacagttcactttttaatttcattatgaatgcaaccatataaaaaccataagttatgaaacagt
cacaatgggaagcttgaaggatcaataaaacaaatgaagggcctttccataccaaggtctgagaggacttacaaaaacagtacaaaacacccaagccaag
tgagcaacccttttgttcctcaataggatgggtgcctgctcctcataagggctagacaagggatactggtgataccatacacattgcaacaggacagaaa
acccaccctgatagtgtccattagttaaaaaaaaaaaaaaaaagtcctgcaaaaaaaaagtgaaagggaatgcacactttggagcttaacactttgctgc
cagcaggggctgaatacttttgcagtgtatacccctttaggccactcagtaatcaagtaatgggtttcctattgttaaaatagtctgcaattctagcaat
tcattgactgaatatactaaaaacaaaaaggaatgcctcttgggatagacctgctaaaggcaggaaaacttagggggctcggaaactgaacagatggttt
gaatccattatggatctttatttacatgatgaacccagccaaggagctggatcaatactctagccttaatggccacagtggaagacaagatcagtgcttt
tggtttcccaaaggagctcttgcactctacactgaactgggcttctaaaagttccccatttgtggctaacaacaggggttaaactttgtaaactatggta
aataaaacaccccgctaaaggttatacaggatgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: