XB-CLONE-4350240
Clone Name: IMAGE:7025826
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785650 [+]
taattaatccccccccccccgtcgcacatagaaaaaacaaatatatatataaataagtgcaagcaagccagggatttaagtgtgtaggcgtccatttgca
gagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacacaagccctcagtgcagccatgcctaagagaaagcacgtgaccgct
gctgatgttgcccatgcccaggatgaacccaatatgatatccttcccgctcccgtcctatacaagccccttaaacccttacaccccccttaatagccctt
ttgcccctcccttgttccccccctccccgcctacatctatcccttatttttcccccagagttacccctttataccaatgattataactatcagatgcacc
ccataacttgatatttattaaaaataaaaacaaggttctcctaaggagggcctatgattacgcatttacccgttttttcttccaccctcttaccttgttt
ttcatctagctccgccttacatttattggaaattttgacattcccatttttcccacctcgcgagcgaattatagacacgcttttaccgtttttattccgc
cccgcctatttcgcaaagaaataaccttcatattgaacgagtgcatctgtcgttctgctttttttttttttttatttaaggtggaagaatcatcaaccgt
cgaataatattatatttttttctgtgccctctcttatcctctaattatatacatagtttatagcacgacgctctgccgtcacccttatattttatataat
attaaccgtatcgggcggttatattttttacactccccccatgtatactacgacgcataacacaattgtcgcctttaatcacacgctcagtaaatatatc
tatagtgaatccgccgtgtccccctatatttctctgcatcattactttctgcccgtaaccgcaatacccgctttattttttttttctgctccaccacacc
ccacgcagtatagtgccaatcgcacttagatacatcttatacttttaacttaatagtatataagagaaacactttctcactatacccccctcccccacct
cgttgtt
3' EST: CF785651 [+]tttttttttttttttgttgttttcttttgtagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaacatgagt
acaaagaacagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgttcgaccggcagcctgcatag
agtactagccgttttgcaacaaaataaaaaaaacattttagactgctcaaggcttgcactccccacagtaactggtttgtggtgtagtattaactggtat
agtattcatagcctttacaaaccatacaggggcaatgtaccaaatcataagtgtttaaaaaaaattttttttacaacaaaaaaatggtggggattccctc
ctattttaaaaatgtgttttctagaattaaactactaaaaaacttgcatttacaaaatagttgataaaaatattcctctgaattgtacaagggaggtaca
ggaaccactgataaaagactcggtacaagatgaaggttattcagactttgattccttgtcgcctccatcagaagcaggggcctcgtcactttttgtttct
ccgttttcagacggctggtcttccttagcttcttctttgttagcttcctctgtctgtttgccctttgctccctttttaccctttgctggaacttttttct
ccttctcctcaagcttttcctttacgggtgccctggctttttttggctttgggttcagcttttggggggggctgggttatcccgacaaacgttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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