XB-CLONE-4350237
Clone Name: IMAGE:7025825
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Gene:
rplp0
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785653
[+]
taattaatccccccccccccccccccgcttttcaacttcccggagccctagtgtgagggaatatctagtctttaaacccttttgtggcaatccttggaag
cactgacaagatgcccagggaagacagggctacctggaagtccaactatttcctgaaaatcattcaattgctggatgattatccaaaatgcttcattgtg
ggggcggacaatgttggttcaaaacaaatgcagcagatccgtatgtccctgcgtggaaaagctgtggtgctcatgggaaagaacacaatgatgcgcaagg
ctattcgtggtcatctggaaaacaacccagctctggagaaacttctatctcacatcaagggtaatgtgggctttgttttcacaaaggaagatcttactga
ggtcagagacatgttgctggcaaataaggttcctgcctgtgcccgtgcaagaacaataccacctttgctgggatcgttggtgcccgctcaaaatactgga
tggggcccctgaaaataccttccttattctatcgctttgggggatcaacggccaaaatctccagaaggaacactgttgaaatctctggaataggaggtcc
aatctgaatttaagtatctgtgataataataattggggtggcccattcgtaaacccactactcattaaaaaattggtagcactatatgtcccgcagtttt
tgtttggtgggatttattgcccttcctcggattgatgttcaaaaaaatattgggccaccaatctaatttatgtctagaatggggcgaaggttgttatgtc
ccgaaaaggtgcgccaccttgttgagggaagcatgtaatacctctaggaaggagggaagttacaacaaaatgagaagtcaccatactagtatatgttgat
atcg
3' EST: CF785652
[+]tttttttttttttttcttttagagactgtaaaagtctttaattatgaacttggaacaatcaaggtttagtcaaagagaccaaatcccatatcatcatcag
attcctcagactcttcttgcttttcctccttagcaggagcagcagcaggagcagcagccacgtcagcagcgggagcagcaacaacaaaggctgatggatc
tgccaggaatgccttgaccttgtcggccagggggaagctgtagtctgtttcaactgcaatggcaaggacacgcttgtatccattgatgactgagtgaggc
acagaggccacggtgggataaccaatctgaagacagacactggcaacattacgaacaccctcaaggaagcgaacatgcaaggcctcctcagtgatatcca
gcacctctggactatagatgctgccattgtcatacacttgttgaatgattaatccataagaaaatggggaaatattcaacatgtttaagagtgtggcttc
actggcacccactttatctccagtcttgatcagctgcacatcactcaagatttcaatggttcctctggagatttttgtggtgatccccaaagcctggaaa
gaaggaggtctttttcaggggccaaatccagtattctgagcgggcacaacgacccccgcaagggtgctattgcctcctgcaccgggcacaggccaggaaa
ccttattttgcccagcaacatggtcttctgaacttccggtaagatctttcctttggtgaaaaacaaaggcccaacattaccccttgaatggtgagaatag
aaagttttcttccagaagctggggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: