XB-CLONE-4350234
Clone Name: IMAGE:7025824
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Gene:
tmem161a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785654
[+]
taattaatcccccccccccccccccactgactgccttaaagggacagcacagtgtccttcctgcagctgttcagtaggaatcacatggctggtgagattt
aattcactctttcaagcaggactgacttactgaacagtaatcatggccattatgggagtgcagatggtggtcacattgttagtggcaagtattatgcagc
gggtgtttcctcactactcctttggcatatggttgctgtgtaatggcagtttgtttcgtttctaacgtccaactgagtaggaaccgcgcacttttactgg
gaaacaaaaacccaatgcctatacacaaacgcggacatatggggtgaccgatgatctaccgctgattgtccactacgacatagacttcacgcttggatac
ccgcgctaatctactactattgtatgctcgtcgatgctttcgctaatttccttggagttaccaatgggagttattatgatttcttcgcctgttagaaatt
ctaacaaaattaaagcatataatctgtttgttctgtgggagacccttaccatttttatcgcgttgttccggggcaataggtctcccaaaactataaccat
ttatatcccgcccccccgatttttcccctatagtgttgtcgtgcccttagcgagaggcggccccacgcggatgtccgcgttttttttttttttatttttt
gtaggtgagggcgtcgtagattaggaganaagatatatttattgttaatgatttgaaagagaagcagcactctctcgcctcgcttgtgccgccatgagaa
tttactataggagtcggttcttcttctggagncatatgtttttattataaaaacaaaaggacgcgttgtgaggactgtagatgtaaaacttgttatgatc
accaacccccttgtatggctctcgcgcaatatataagcatccagatacctccgcccgcattgaaaattttcttccgttccactgtttatctatcatggct
ctaaataccgtactctcatgcntacaacccctaaatatacgtcggcacgctctgattgttttgggtgggcgcccactcatgcgcgcgggtgacgagacaa
aatttcacgctagttatatattgccctgtccaccaacnaagcacccgtcgaaaactgaatgtataacacttctgggggaacaaatatattcatacgtttc
tggtatccgtctttttgtggtgcgccagtggggcgaaagagagagtatttttattgtcatcatcacctcctaaacaccaccccaattctttn
3' EST: CF785655
[+]tttttttttttttttattttttttttttgtttatagtgatatcagaccattcttctacagcctgattgtgattagacttgatggacagattgactctcaa
tcactactgcacccaagtcaatgataagaataggctcccgtgattaaaccatctgaggactagctaaatcaaaaccaaagctgggtcttgtctccatgta
ctgtatttcaaatataataagcttgctttgtactaattctgttcatagttattccaggtcattgataatacataatgcttgaatttcttcttgctttttt
atgaaagatggaatgtccttcactgtactacagccatcaatgtatttgtattcttaagtatataatagaaaaggaaaaaacaataaaatgactaggcact
ctttatcagcatttgtacaatctttcaaaaacacagcagtctttccttttctatagtgtacacatatacaccatcagtagcaattgtaccccatccactt
tgttaatgcgtgtttttcaaaaataaaatatgaatacagaatatgaattttacatttaaatttttttcccatatgcaaaagaaaaggatcacagctcata
gttataaaaagctaaactgcccaaaaatggcatatattttgatgtaactcatatatccataatgacaccagtatattgcctctgtacattcaactgaaac
acaatgcattacataaaagcagtcattctaatctttacaaaaaatgcatagtcagccttttcaactatggaacatgcagctcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: