XB-CLONE-4350231
Clone Name: IMAGE:7025823
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Gene:
eif4a3.1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080171 [+]
CGTCCGCGTCGCGCTGCTCTTGCACATTCGCTAAGGGAAAATGGCGGCCGCAGCAGTAACAGCAGTTGCCGGAGTGACTAGCGGGTCAAGCCGTAAGCGG
CTTTTACGGGAGGAAGATATGACTAAGGTGGAGTTCGAGACCAGTGAGGAGGTGGATGTAACGCCAACTTTTGATACGATGGGGCTGAGGGAAGACCTTC
TGAGAGGCATCTATGCTTACGGATTTGAGAAACCATCAGCAATCCAACAGCGAGCAATCAAGCAGATTATCAAGGGAAGGGATGTGATCGCACAATCACA
GTCTGGTACAGGCAAAACAGCAACCTTTTGTGTTTCTGTGCTTCAGTGTTTGGATATTCAGGTCCGTGAAACCCAAGCCTTGATTTTAGCACCAACCAGA
GAGTTAGCTGGGCAGATTCAGAAGGTGCTACTTGCTTTGGGTGACTACATGAATGTGCAGTGTCATGCATGTATCGGAGGCACAAATGTTGGAGAAGATA
TCCGGAAATTGGATTATGGGCAGCATGTTGTTGCTGGCACACCAGGACGTGTTTTTGATATGATTCGACGCAGAAGTTTAAGAACTCGGGCCATTAAAAT
GTTAGTGCTGGATGAAGCTGATGAAATGTTGAATAAGGGTTTTAAGGAGCAGATTTATGATGTCTATAGATACCTGCCACCTGCAACTCAAGTTTGTTTA
ATCAGTGCCACGCTGCCACATGAAATCCTGGAAATGACCAACAAGTTTATGACTGATCCCATCCGTATCCTTGTGAAACGTGATGAGTTGACGCTGGAAG
GCATCAAGCAGTTTTTCGTGGCAGTGGAGCGAGAAGAGTGGAAGTTTGATACTTTGTGTGATTTATATGACACATTGACTATTACGCAAGCTGTGATCTT
CTGCAACACGAAGAGAAAGGTAGATTGGTTAACAGAGAAGATGAGAGAAGCAAACTTCACAGTTTCATCAATGCATGGAGACATGCCCCAAAAGGAGAGA
GAGTCAATCATGAAAGAATTCAGATCTGGTGCAAGCCGAGTCCTCATTTCAACTGATGTCTGGGCCAGAGGATTGGATGTTCCACAGGTCTCCTTGATTA
TCAACTACGATCTCCCCAATAACAGAGAATTGTACATCCACAGAATTGGCCGATCGGGACGATATGGAAGAAAGGGTGTTGCCATTAACTTTGTCAAGAA
TGATGACATCCGTATCCTGAGAGATATTGAGCAGTACTACTCGACCCAAATTGATGAAATGCCCATGAATGTTGCTGATCTCATTTGAAAACCTGAAAGA
TATCAAACTTTTTGGAAAAGGAGTTATGGGGAGGATCACATTTTTCAACATTACAAATTCTTTTCATTATTGCTGAAAGTTTTATTTTGTGGACTTTTAA
CTGTCTTTATTTAACTGTTTATGAAACGCTGCACCCAATCCATTGTAAACCCTGTAAATAGTGCAATTGGTGTCTTTTCAATAAAAAAAATCCATTCAAA
AAAAAAAAAAAAA
5' EST: CF785657 [+]tataatccccccccccccccgtccgcgtcgcgctgctcttgcacattcgctaagggaaaatggcggccgcagcagtaacagcagttgccggagtgactag
cgggtcaagccgtaagcggcttttacgggaggaagatatgactaaggtggagttcgagaccagtgaggaggtggatgtaacgccaacttttgatacgatg
gggctgagggaagaccttctgagaggcatctatgcttacggatttgagaaaccatcagcaatccaacagcgagcaatcaagcagattatcaagggaaggg
atgtgatcgcacaatcacagtctggtacaggcaaaacagcaaccttttgtgtttctgtgcttcagtgtttggatattcaggtccgtgaaacccaagcctt
gattttagcaccaaccagagagttagctgggcagattcagaaggtgctacttgctttgggtgactacatgaatgtgcagtgtcatgcatgtatcggaggc
acaaatgttggagaagatatccggaaattggattatgggcagcatgttgttgctggcacaccaggacgtgtttttgatatgattcgacgcagaaatttaa
gacctcgggcccttaaatgttagtgctggaagaagctgaagaaatgttgaaataagggtttttaaggaagcagaatttttgaatggtctttttgaaaacc
ctggccccccggccaaattcaaggttttggttttaatacaagggcgcccacgccttgcatcaatttaaaaaaccccgggacaaaaggagaccctaccaca
acttcttaatgtgacaccggaattcccccctattcccggtaatccacttgtgggttgaaaaaaccatcgtgttatcttatgttat
3' EST: CF785656 [+]tttttttttttttttgaatggattttttttattgaaaagacaccaattgcactatttacagggtttacaatggattgggtgcagcgtttcataaacagtt
aaataaagacagttaaaagtccacaaaataaaactttcagcaataatgaaaagaatttgtaatgttgaaaaatgtgatcctccccataactccttttcca
aaaagtttgatatctttcaggttttcaaatgagatcagcaacattcatgggcatttcatcaatttgggtcgagtagtactgctcaatatctctcaggata
cggatgtcatcattcttgacaaagttaatggcaacaccctttcttccatatcgtcccgatcggccaattctgtggatgtacaattctctgttattgggga
gatcgtagttgataatcaaggagacctgtggaacatccaatcctctggcccagacatcagttgaaatgaggactcggcttgcaccagatctgaattcttt
catgattgactctctctccttttggggcatgtctccatgcattgatgaaactgtgaagtttgcttctctcatcttctctgttaaccaatctacctttctc
ttcgtgttgcaaaagatcacagcttgcgtaataagtcatggggtcataaaaatcacacaaagtatcaaaacttccactctttttcgctccaatggccacg
aaaaactgccttggatgcctttcaggcggcaaactcatcaacgtttcaaaaaggaaacgggatggggaatcagtcctaaaacttgggtgggccattttcc
aggaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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