XB-CLONE-4350225
Clone Name: IMAGE:7025821
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Gene:
slc18a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785661
[+]
taattaatcccccccccccctgacagggttacatgatgaaatccatttggctgcacatctcatgctaactggatcgggacacagaaattcaccaacatta
gtacaggaggagttatcgggtccataacaaaatggcaagatgttccccatgttgtcagtggttgaagaaaacatgggagtcccgggctccgatcctgttg
gtggttgcagtggttatgtttgtggattgcattctgttcactattattgctccaattgccccagctttactttatgacattgagtatgggagaggaaaca
gaacatttaagaactcctccggtcagtatcctgtctccaactatgattccatttttaatctttcacaccttgaagactcaagaaaaggaccttaccatga
ccagatcattaatattactgagagtcctggagataaaaagaactgctacgaggggaaggactttttaaacgaagagaatgtgcgagtgggtcttctcctt
gcaattaaagcctttcttcagcttctgtttaaccccattgtgggtaaactcgttaccaggattggatatgatgccccaatgttttttggtttcgtaattg
tggtcgtctccactcttttgtttgcatttgccaactcatatgcccttctttgtgtgggccagggggttccagggaatttggatcatcctttcctatgggt
gccggcttctgggcacacgggcacaaagtgtttcccggatgatgttcgaaagaagaaaaagccaggggtttagcccatgaaatggtgtttgccaattggg
gggtgcctccccta
3' EST: CF785660
[+]tgaggagaaccctgtagggtgtttcaggtacactcaatggggaaaactttgtaggcttaagtacacatcactgcagtgctccatcaataagagctaatgc
agctataaccagaaacggagaagattttccaacaaattcatacatggcagaaccaaagggaggcccagcgagcaccccaatggcaacaccactcatggct
aaacccatggcttttcctctttcgacatcatccggaaacacttgtgccagtgtgcccagagccggcaccatagtgaaggatgatccaattccctggaacc
ccctggccacacaaagaagggcatatgagttggcaaatgcaaacaaaagagtggagacgaccacaattacgaaaccaaaaaacattggggcatcatatcc
aatcctggtaacgagtttacccacaatggggttaaacagaagctgaagaaaggctttaattgcaaggagaagacccactcgcacattctcttcgtttaaa
aagtccttcccctcgtagcagttctttttatctccaggactctcagtaatattaatgatctggtcatggtaaggtccttttcttgagtcttcaaggtgtg
aaagatttaaaatggaatcatagttggagacaggatactgacccggaggaagttcttaaatgttctgtttcctctcccatactcaatgttcataagtaaa
gctgggggcaattggagcaataatagtggaaagaatgcaatccaacaacataaccactgcaaccaccaaccaggatcgggagcccggggactcccatgtt
tttcttcaaccactggacaacatggggaacatcctgcccatttgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: