XB-CLONE-4350217
Clone Name: IMAGE:7025817
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785666 [+]
taattaatcccccccccccccgggcagtgggttcattttcatgatgccagactgggaacagttcataggtctctgctgccctcccccttgtgtacagaat
gttctgcacctaatgttgtgacaactgactgcttcctaatatgacttcatgagtgatatttatttttttactctgtttttaaacaatttaaatgtaaatt
cccaccaatctcacttgagtcattgtggtctgtcattaagtggttttattatttgtgacttctctcaagttggagtctggccatacgttgatgtaaccct
gggaccaaacgattggatcacattgatgggagacaggctgtggggacacaaaccacatcaaaaagctgatgcgctactcatcccatttcttaatctgcct
gatcagcattctggacagttttgggcccaaataatggttggtttatgccccatctaaaaggcctcattacatgggtcagataaggcttcctattttttca
acatttttatggggcccccttggtaatgcgcccatcctttttaaatggaaaattagggcataactttacctccccaaatgaaccattgggggggtggtcc
ataaaacaaatactttagccacgcccacttgatgattattattaataagcgacccctttataataataaaaattggggctatactaatacctagtcctct
taatcctttatatattggggtgaaatatatttggtcgaataacctaaaatgtgggggtaaaaggaaaaaagagggccatattgtgttttagtaatccacc
aaactaagaaaaatacttctgacacctcaatttcctgatgtggtgttatacatattaagaaatttttgggcccttcataaatccccagaatcttaacagt
ttgtggtgccgtctgaagatacacccacctgttagaataattcataataatttaatgccctactgtatcttctat
3' EST: CF785665 [+]tttttttttttttttctattttttttttttttgttttttatagaaatacccgagtatcgttgttgtatgtgcaaatagacctagacctatttgaagctac
gtgtgcagcgggatgttccctcagcaaacacctgttaatcctgaatgtgcagagacgcattcccatgcaaagacaccctgcgtgcttgttttgaaatatg
ataatggcaatatttgctgctaggtttgtttgcactatcatattataccaacaagcatattcaggtcctagccctcaaggtgcacacatagaggaaagta
gagaaggataggaacaggggacagacaaatagtgaataggcactctttgtaccttgatggtaagctctgtgggtcagccctgccttttttgccatttgta
tccatgtaccctataagttaggctgttgtgccacttctccaaggccttaaatgtacaggctgcaatttgttatgcaccccatggtcttttactaagtagc
ctatccccttaaagatggccatacatgggcccataaaagctgacaactggaagcctatctgcccatgtatgaggcctttagatgggcctaaccaaccatt
atctggcccaaaactgtccagatgctgatcaggcagattaagaaatgggatgagtagcgcatcagctttttgatgtgttttgtgtccccacagcctgtct
cccatcaatgtgatccaatccgttggtccccgggttacatcaacgtatggccccgactccaacttgaagagaaatcccaaataataaaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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