XB-CLONE-4350208
Clone Name: IMAGE:7025814
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Gene:
aph1a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785671
[+]
taattaatccccccccccccctgggagtttcctgcctccttcgacatcgaccgagaatttcctttctgttcgacgtcggtttgacatccgtctgagagta
gctttgaggaacgctgatactaatacaactgggtggcgctgctattggactatacctctggcctctgggcacggaacggattgtgtctccttcctttcag
ctttcacatctagaacagtccaaggtgagactgttcctcaagtagaggggacacagttgcactgcattttctttttttaatctatctcctttcccaaccc
ttacggcaaatgtctacactgggttccctgcagccattcttgtcactggaaggagggactcctcaataaaatgtgttttgattggtcggactaataaatc
gggagtccgtctaccaatatcttatccttatgacctgctgcttgggctaacccctttattggaaggccccctaaaccaatttgagatgggtaacaacccg
tcttcccttttggtttttaagaataaaaacacttttttggccggcggacgggaagatccgccggccttcagcggggtggggggtgacatgaatgtggaat
taaaccgcgcggggaattctcagataaagtgccttcgtggcgcatataaatataaatacctccccgcgccgaaaaaaaaaatttgttctaactagaatat
gggcaacgcctagacattaccaaacccgttttgcgacaatacgatcattcactgctttttcaccgcccattaaaaaaaataccgtggtggatcgcacttc
cgcggtgctgattacattcatatatttaatgtacgcgaagccggcgcggcccccggcacgaggatttttattttgtatgagggtgacgcggtggatccgt
gcgctttatcggagaactactaatgctgtacagccactacactcgattaaatttgtgctgcgatagtatcgcaaacttatttcgactatgccacagacat
agcgtgtgaactagagngatgcgccccgccgatatataggagtgcacgagcacgagacgagcttatgaatacgatatggtanataacnctattttaacaa
cataactagatatgcgacgaggacaggtgtagtggctattgttagtgtcttgctcgcgttgtgctccgcttgtatcgtttcacatcgcgcgtaacttgga
attctgttgaaactgagatagctcagttaatgtgcaccatcgtgtgagcaatggagagaaatgaatttatatctcattctccaccagacgcaacgggaat
a
3' EST: CF785672
[+]tttttttttttttttcagtgctgccattagtttagcatgtctgtaccaactcaaatcaaaatacctcaatggaatatgttacacatcctcactggttatc
tctcaggttcagcttgggcatgtgtatacagatcaatgcaggacatagaaactgccatgggcatctgaacacatatgggtatgtctgccaaccacaaagc
ctgctggtagattaaaaagacattttgtattgtatcagtaaaagtcatttacattttcggcgttattagccaaccgctgattccgtttatcagcagcaga
aacgtgaaacagaattaagtcacgacaaagtattaatctggaacaaagagaagggtgtggcctctgagttgctcggggcttgcatgaagggttaaccagc
tgcagtcataatgattaatattggtatacagattaccgatttattagtccgaccaatcaaaacacattttanttgaggagtccctccttccagtcacaag
aatggctgcagggaacccagtgtagacatttgccgtaagggttgggaagggggatagattaaaaaaagaaaatacagtgcaactgtgtcccctctacctg
aggaacagtctcaccttggactgttctagatgtgaaagctgaaaggaaggagacacanatccgttccgtgcccagaggcccagaggtatagtccaatagc
agcgccacccagtttgtatttagtatcagcgttcctcaaagctactcttcagaccgatgttcaaacccga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: