XB-CLONE-4350205
Clone Name: IMAGE:7025812
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Gene:
rps3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785673
[+]
taattaatcccccccccccccgcttttccttccagcggcgcttcgcaagatggcggtgcagatctctaagaagaggaagtttgtcgctgacggcatcttc
aaggctgaactcaatgagttccttactcgggagctggctgaagatggctactccggtgtagaggtcagagtcaccccaacccggactgaaatcatcattc
tcgctaccagaacccaaaatgttctgggtgagaagggcaggcgcatccgtgagctgactgcagttgttcagaagaggtttggattccctgaagggagtgt
tgagctctatgctgagaaagttgccacaaggggtctgtgtgccattgcccaagccgaatctctccgttacaaactcctgggaggtctggctgtgaggaga
gcttgctatggagtcctccgtttcatcatggagagtggagccaagggttgtgaggctgtcgtgtccggaaaacctacaggccagagagccaagtccaaga
agtttctagacggtctgatgatccccaatggaaaaccaaataaatactacccggatactgttgtaaaccatgtggcccttaggaaaggtgtccttggaaa
ttagggaaagaatgcgcctcccctgggaaccaggtgggaaaaatatgaacccaaaaagccccttggccggaccccctcaacattgtattaaccctagaga
agaaaaatcttgcctacaaccccctaaatttttgaattataaaaggaccccagacccaccatcacgtctctatgcaaccccggccaagggcgaatcaccc
cgggttacaaacagtctttattggggaccggaaaagacctgggttattccttacacttgtactgg
3' EST: CF785674
[+]tttttttttttttttggattttaatttttttattttagatgttctgatactgtatccaaaatctttgaatccaggacttcagcccattatgctgtgggca
caggctgtggcatggcgggtggttgtggctgatctggcttggctcccttctgctcagagatgggggttgtaggcagaatctcttccttgggctcaacaat
gctgacgtggtcaggcaggggcttcttgggtccgatctttccacttggatcccagggaagcataatctttaccttgattccaaggacaccctgcctgagg
agcacatggcgtacagcagtatccacgtagtaattaactggatctccactgtggatcatcaggccgtctacgaacttcatggacttggctctctggcctc
ttagttttccggacacgacaacctcacaacccttggctccactctccatgatgaaacggaggacaccatagcaagctctcctcacagccagacctcccag
gagtttgtaacggagagattcggcttgggcaatggcacacagaccccttgtggcaactttctcagcatagagctcaacactcccttcagggaatccaaac
ctcttctgaacaactgcagtcagctcacggatgcgcctgcccttctcacccagaacattttgggttctgggtagcgagaatgatgatttcagtccgggtt
gggggtgactcttgacctctccaccggattagccatctttcagccagctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: