XB-CLONE-4350202
Clone Name: IMAGE:7025811
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Gene:
ybx1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785676 [+]
taattaatccccccccccccccagttcgatcggtagcgggagcggagagcggacagcagggagctcgaaaggcagcagccccaccgccggcctgagttac
catcaacaccccggggggaagttaaagcgagccaaccgccggcccgagtcaccacctccgaaagcagccaacttacaccatcaaagacacaatgagcagc
gaggttgaaacacaacagcagcagacagtcgcattggagggcaaggccggccaggaaccggcggccaccttgggggagaagaaggtaatcgcaaccaagg
ttttgggtacggtcaaatggtttaatgtgcgcaatggttacggcttcattaacaggaatgacaccaaggaagatgtgtttgtacaccaaactgccatcaa
gaagaataaccctaggaagtaccttcgcagtgtgggagatggtgaaactgttgagtttgatgtagtggagggtgaaaagggtgcagaggcagctaatgga
aacttggtccagggggggttccactccaaggcaccgaatatgcttacagagccttagtcattttccaggcgctaaaaaacattcaacaaaaaggcccttc
cactgaatcctaccccagttaaaatttacccaatattaatagaaaagggggatgattaataatctatttgggcgaacggcaaaattgccaacctagatta
ggaaaaagggggagggttccaaaaaatcatgataaacagatcccaatatccaattcacgcggaaaacaccaggttttcatagccctaacaccaatattgg
tcgcgataactaccatgtattgtgtgggcgccgatgtctcttccataagttattttacaataaggggccatccccgtgtttactccgaggaataaaaaaa
gtgtgtgaataatatgggcgatcattattt
3' EST: CF785675 [+]tttttttttttttttatgttgagacctttattaacagatgcttgcagtttgttgactcttgaaaaaaattaggtgtacgcttattacaaattaaaaatga
ggttcttaaaaatcttagtttgaccagataggaaacaatttaaaaacctttgaaaggtacattgagaaaaaatatgtttttaaaaaattttcattcccaa
aaggagcttttagaaaaaaaaacataaaaaccatctctgcatttacccatgcaggatgaagttatctggtcaagagcaaaaagcaagcacttaaggtctt
cagttccaattctttgatcatttcttattgctggaatttctgtcttgacattttgatgaccaaaaaccgtatggaggaaaagatggtaagacagtattta
atagtccacgccctgctcaacctcagagaccggagagtgttacgcaccgaagtttttcggaggcctggggacgtctttaaaaatcttggaggtataaagg
attttatgaggccgtctagcctccagttatgataaactatatgcgctaattagcagaaccgagtaatggggcctggcatagcatccaggataactcacac
acacccaggatagtatagaaaagtcgagacgttacatttcaccacacataccagcatagataaggtacaatagagacccaagagaaagctccacaagttc
tgacaacataattgggttactataagagacgtagctggacaaagataagatattgcagcccgaaagatgcgtaacatgtatttgaagcgagctatgtgac
acaaataaccgttagacagagtgtcacgtcctagattgtagactcatatcattacgttatgtaatagagcaagataaactgtcagagaaaaatgggatcg
gctatatatatgaacncgtagttagtgagcaactactaacagnag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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