XB-CLONE-4350197
Clone Name: IMAGE:7025807
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Gene:
tma7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077652 [+]
GGCGTCGGGCCTTGACTGGGATTGCGCGGCAGATTACAGAGAGAGATGTCTGGAAGAGAAGGAGGCAAAAAGAAACCTTTGAAACAGCCCAAGAAGGCTA
ACAAAGACATGGACGAGGATGAGATTGCATTCAAGCAGAAACAGAAAGAAGATCAGAAGAAACTAGACGAAATGAAAGGGAAAGCTGCCCAGAAAGGCCC
CCTGACTGGAGGCGGCATAAAGAAGTCTGGGAAAAAATAATCTGCTTTCACCCGACAATGGAATTCCTCTTCTAACTGATTTCTCAAGCGCCGGGCCCTG
ACCCTGGCCATGGAGCGACTGCCGGCCCCCAACTTATTGTATGTTTCCGTGTACAGTGTAATAAACACACGTCAGTTCTTTTTTTTAAAACCTGTGATAC
CCATGATTTGAAGTCAGCAGTGGAATATTCCAATGACCTTGTCTAACAAAATGACTCGCTCCCGGATGTCGTCTTTCAGGTTCAGTAGATAGTTCTGTGT
TTGGAAGAGCAGAATATTAGATATGAGTTGTCGAAATAAACACTTATTGCCAATAAAAAAAAAAAAAAAA
5' EST: CF785679 [+]taattaatcccccccccccccggcgtcgggccttgactgggattgcgcggcagattacagagagagatgtctggaagagaaggaggcaaaaagaaacctt
tgaaacagcccaagaaggctaacaaagacatggacgaggatgagattgcattcaagcagaaacagaaagaagatcagaagaaactagacgaaatgaaagg
gaaagctgcccagaaaggccccctgactggaggcggcataaagaagtctgggaaaaaataatctgctttcacccgacaatggaattcctcttctaactga
tttctcaagcgccgggccctgaccctggccatggagcgactgccggcccccaacttattgtatgtttccgtgtacagtgtaataaacacacgtcagttct
tttttttaaaacctgtgatacccatgatttgaagtcagcagtggaatattccaatgaccttgtctaacaaaatgactcgctcccggatgtcgtctttcag
gttcagtagatagttctgtgtttggaagagcagaatattagatatgagttgtcgaaataaacacttattgccaataaaaaaaaaaaaaaaag
3' EST: CF785678 [+]tttttttttttttttattggcaataagtgtttatttcgacaactcatatctaatattctgctcttccaaacacagaactatctactgaacctgaaagacg
acatccgggagcgagtcattttgttagacaaggtcattggaatattccactgctgacttcaaatcatgggtatcacaggttttaaaaaaaagaactgacg
tgtgtttattacactgtacacggaaacatacaataagttgggggccggcagtcgctccatggccagggtcagggcccggcgcttgagaaatcagttagaa
gaggaattccattgtcgggtgaaagcagattattttttcccagacttctttatgccgcctccagtcagggggcctttctgggcagctttccctttcattt
cgtctagtttcttctgatcttctttctgtttctgcttgaatgcaatctcatcctcgtccatgtctttgttagccttcttgggctgtttcaaaggtttctt
tttgcctccttctcttccagacatctctctctgtaatctgccgcgcaatcccagtcaaggcccgacgccgggggggggggggggatttaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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