XB-CLONE-4350184
Clone Name: IMAGE:7025800
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Gene:
actc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785686
[+]
aattaatcccccccccccccgacccagccaatttaatcacagcaaccagctctcctgtgctgaccagaacctaacctgccactatgtgtgacgacgagga
gactaccgccttggtgtgcgacaatggctccgggctggtgaaggctgggttcgctggggatgatgccccacgtgctgtgttcccctccatcgttggtcgc
ccccgtcaccagggtgtcatggtgggtatgggtcagaaagactcctacgtaggtgatgaagctcagagcaagagaggtatcctgaccctgaagtacccca
ttgaacacggtatcatcaccaactgggatgatatggagaagatctggcaccacaccttctacaatgagctgcgtgtggcccctgaggagcaccccaccct
gctcactgaggccccactgaaccccaaggccaaccgtgaaaagatgacacagatcatgtttgagaccttcaacgtcccagccatgtatgttgccatccag
gctgtgctgtccctgtacgcttctggtcgtaccacaggtatcgttcttgactccggtgatggtgtcacccacaatgtccccatctatgaaggttatgccc
tgccccatgccatcatgcgtctggacctggctggtagagacctcacagattacctcatgaaaatcctgactgaacgtggctactcttttgtcaccacagc
tgaacgtgaaattgtccgtgaacatccaggaaaagctgtgccatgtggcttttgaactttgaaaaagac
3' EST: CF785687
[+]tttttttttttttttgaacgtaaccgcttgtgttttatttagaaagtaataaatatgatggagtgtaaacaaactgtacagtgactgtccaggtcttgtt
ttcttcgactttgaggtactttgtatgtgttgcttggaggagtgtgcgtaatgtttagaagcacttcctgtgcacaatggaggggcctgcctcatcgtat
tcctgtttgctgatccacatctgttggaaggtggacagggaggccaagatggagccaccgatccagactgagtatttacgctcaggtggggcaatgatct
tgatcttcatggtgctgggagccaaggcagtgatttccttctgcattctgtcagcaataccagggtacatggtggtaccaccagagaggacattgttggc
atacaggtccttgcggatatcaatgtcgcatttcatgatgctgttgtaggtagtttcatggataccagcagattccataccaatgaaggaaggctggaag
agggtctcagggcagcggaaacgttcatttccaatggtgatgacctgaccgtcgggaagctcatagctcttctccagggaggaggaagaggcagcggtgg
ccatttcattctcaaagtccaaagccacatagcacagcttttccttgatgtcacggacaatttcacgttcagctgtggtgacaaaagagtagccacgttc
agtcaggattttcatgaggtaatctgtgaggtctctaccagccaggtccagacgcatgatggcatggggcagggcataaccttcatagatggggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: