XB-CLONE-4350169
Clone Name: IMAGE:7025794
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785695
[+]
taattaatcccccccccccccacattttcacaccctcccctacgctccctctgcatcagtgcttggggtttatacacctaacttatttttagttcaacca
tgttctctaaggaccaggccagaaaatcagtgtaatggcctagtaggctttggtaattcagcaggcaagggttgtgctctacactgaccccaattattcc
tttctattcctgtttgctgtatattccaccacactttggacatgaaagaagctggcttaatgtcatgacaactgttttgtaattaaaagttataactgca
aataaattatccaaagtgtatgtattcctatggattgctcctgtcatttttcaccaaaagcaggacataaggtcactagcatatttgctgtattgattta
attcctgcaggtactaaattagtgatggtttatgtagctttatattgtaagtagggtgggggtaactactttaagtaaaaattatgatttccattgcatt
cgcttgccagtgactggaattgtgtcaggatttttaaataatgtgctgacagttttacttttaaaataattggggcataagggctcacggtttttttcat
gcctgtggtggtttccgcctgggttacatcccccccccccaaaacgtacaggctaaatctggaaaaaaaatggaccactgaaccccctgggaccccccct
gggagaaattttttgngaatcaaggcaagggccagtgtggaaaacacctggggggcaagggaaatggattgtgaaatggagggaataaaagaaactgcc
3' EST: CF785696
[+]ttttttttttttttttgtttcattttcattatgtttattattgtttcatcattccccttattacataaaaatatttgaatacatttctgtgtatataacc
tatgttgtacaagttttattatacttttcttctttatttttctttttattttgtcacccctttatcacctccaactagtacggtccaatgcatacacctt
ggcgggttctactaacatacaggtataaattgtaatttgagcatctaataatgcacacctatatcaggggcactaataaagtctacctagctaactggca
ttgcataggtagccattggtgtatcaactttcatatagagtaccaagagcttttatgaccaacaagtgtttgcccccaaacattaccctaatttgctctc
aagcttggctttaagtggcatataaaatatgaaaataaattatactcaaatctcaccctaattgtcctttagactgagccccgaactatgtaacgcagtt
ctttatccatcattcacatcactccctgccccagtgttttccactggcccttgcattgatacaaaaaatacctccaggtggggtccaggtgttagtggtc
cattctttctccaaataacccgtatgtttttgggtgtggaatgtaaaccagacgaaacccccaaccttgggaaaaaacagtggagccacttattggccca
atttttttaaaaagtaaaaacctgttccgccacaattattttaaaaaattcttggaaaccaaatttttcgagtccacctggggcaaagcggaaatggcaa
ttgggaaaaaatccccaaaattttttaacccttaaaagggtagtttttacccccccccagcacccttaccctaataaaaatatttagaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: