XB-CLONE-4350162
Clone Name: IMAGE:7025790
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Gene:
hmgn1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785699 [+]
taattaatcccccccccccccggactagtctttgtgagtactccatctgcaggagggctcgcacatagaaaaaacaaatatatatatatataaataagtg
caagcaagccagggatttaagtgtgtaggcgtccatttgcagagaaggctgctaagcgttcccgttgcttctgtctttggattcaagcggtgcacacaag
ccctcagtgcagccatgcctaagagaaagcaggtgaatgctgctgatgctgcagatgccaaggatgagcccaagaggagatcggcacggttgtcggctaa
accagccccaccaaaagctgaaccaaagccaaaaaaagacaaggcacccgtaaaggaaaagcctgaggagaaggagaaaaaagttccagcaaagggtaaa
aagggagcaaagggcaaacagacagaggaagctaacaaagaagaagctaaggaagaccagccgtctgaaaacggagaaacaaaaagtgacgaggcccctg
cttctgatggaggcgacaaggaatcaaagtctgaataaccttcatcttgtaccgagtcttttatcagtggttcctgtacctcccttgtacaattcagagg
aatatttttatcaactattttgtanatgcaagttttttagtagtttaattctagaaaacacatttttaaaataggagggaatccccaccattttttttgt
tgtaaaaaaaaattttttttaaacacttatgaatttggtacattgccccctgtatggtttgtaaaggctatggaatactataccagtttaa
3' EST: CF785700 [+]tttttttttttttttaactttttcaaccaggttttattggtttggtgatcagtacacagacactatgaacaatctgtacatttttttttcctttttaaaa
ctctagtagattggaaatctaggaaaaatatgaaaaaagaaaaaaaataaaaacaatgtcgttgaagtcattggaatccattttctagcggctttccggt
ttttttttttttttttttaaagttcagagagaagttgtttcgtaaaaaatctagcacccctcgtaccagcatctttgatgcaaataattcaacccatttc
tcggccaagaaggagcgaacacgcataggtgccaccttggcaggaaagagatgcaaacacatgaaccgccgtgtcctttttgtttttctccctacttcac
atcacactcactacacactcaaagaagtttccccagaagagattcacagcacttttacaaagttactgaaaataaaataccactacccagaaaaaaaaaa
ataaaatggtaacatcacagatagtacacaattctttagagggaaatccgtcaccttgggagatttattagtttaaaagtgtaaattgtacgcaggacaa
catgagtacaaagaacagttaaaacgttaagacattttaatagccgtgatctttgcgccagttgtaccagtatcgggcactgcctgtttcgacggcagcc
tgcatagagtactagccgttttgtaacaaaataaaaaaaacattttagactgctcagggcttgcactccccacagtaactgggtttgggggtgtattatt
aaccgggtaaataattcatagcccttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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