XB-CLONE-4350156
Clone Name: IMAGE:7025788
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Gene:
atp5f1b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785703
[+]
taattaatcccccccccccccgtgcactgctgctgctctccgctgctgacgccgccatgttaggggctgtaggacgttgttctgcgggggcactgcgggc
tctaaagccagcatctaacccagttcagctcgggcagaaccttctcagatgttcccctgcagcgctgcactcccggagagattatgcagctcagacttcc
gcagcagccaagcctggaacagcagtcggccgcattgtggcagtcattggtgctgttgttgatgtccagtttgatgaaggcctcccacctatcctgaatg
ccctggaggtccagggcagagacaccaggcttgtgctggaagttgcacagcatctgggtgaaaacactgtccgaacaattgctatggatggcacagaagg
tttggtcagagggcagaaagtccttgatgctggcacaccaatcagaattcctgttggtcctgagacccttggaagaatcatgaatgtaattggtgaaccc
attgatgaaagagggcctattactaccaaacagtttgcagccatccatgcagaagccccagagtttgtggagatgagcgttgagcaggaaatcttggtta
ctggcattaaagttgtagatctgctggcaccctatgccaaggaggaaaaaatggcctgtttgggggtgcaggattaggtaaaactgtgcttaattatgga
actgatccacaatgtagcccaagctcatggggggttactctgtgtttgctgggaattgggaaaacgtaa
3' EST: CF785704
[+]cagcaggataaatacccaactcagcaatagcacgagacaatactgtagtagcgtccaaatgggcaaaggtggtagcaggagctgggtcagtcaagtcatc
agcaggtacataaatggcctgcacagatgtaattgaccctttctttgtagttgtaattctctcttgcatggtacccatgtcagtggccagagttggctgg
taaccgacagcagaggggatacgtcccagcaaggcagacacctctgaaccagcctgggtaaacctgaaaatgttgtcaataaaaagcaacacatcttgtc
cctcttgatctctgaaatattcagcaacagtcagaccagtcaaagcaacacgagctctggcacctgggggctcgttcatctgcccatataccagtgcgac
ctttgaggttgtatccttcaagttgattactccagattcaatcatttcatgatacaaatcatttccttcacgtgtacgctctccaactccagcaaacaca
gagtaaccaccatgagctttggctacattgttgatcagctccataattagcacagttttacctactcctgcaccaccaaacagaccaatttttcctcctt
tggcatagggtgccagcagatctacaactttaatgccagtaaccaagatttcctgctcaacgctcatctccacaaactctggggcttctgcatggatggc
tgcaaactgtttggtagtaatangccctctttcatcaatgggttcaccaattacattcatgaatctttccaagggtctcaagaccaacagggaattctga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: