XB-CLONE-4350153
Clone Name: IMAGE:7025787
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Gene:
ilf2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785706 [+]
taattaatcccccccccccccgctagtgtggctgtatcgagggccgagagggtgtccattgtgcgggagtgtgaggaacgaagatctgaaacatgagggg
tgaacgaggacgtggacgtggccgctttggctccagagtgggacctaacacaggtttcagacccttcgtccctcacattccctttgatttccacgtgtgt
gaaatggccttcccgcgtgtgaaacccgcccccgacgatagctcattcagcgagtcactgctcaaacgaaaccaagatctggcccccagcacagccgagc
aggcctcaatcctttctttggtcaccaaaatcaacaacgttatcgacaatttgatcgtagccccgggaaactttgaagtgcaaattgaggaggtgcgaca
ggtgggctcctacaagaaagggaccatgagcacaggccataatgttgcagacttggtcgtcatcctaaagatccttcccacactggaagccgtttctgct
ttgggcattaaggtggtcgagaccctaaggacgcaggatccagctgaagttctcacaatgctgacaaacgagactggctttgagatcagctcagcagacg
ccacggtgaagatccttatcacgactgtgccgcccaatctgcgcaagctggaccccgagttgcatttggacatcaaagttctgcaaagcgcgctggccgc
catcaggcacgcccggtgggttcgaagaagaacgcccttccatttctacagtggaaggtttttaatcccggttggttgaaaaaacttggaaggaaatcgg
gtttcc
3' EST: CF785705 [+]gaccaactgaaatctttaaataagtatcactcctgtgtctccatgctctcctcctcttccgccccctctgggatatcctggttctcatcctcctctccct
ccttcctctcgggggggcttctcatagggctttctcctaaaggggtcacaaaaaaccccctccccaaaaaaaaatttttcccggaggaaaaaaaaccgct
tggcattctcccttccagggccccaaaaatctttccggaaaaacccccccctgggggaaaaaaaaaaatggggaaccaaactttttttgggggccatttg
tttaacaaaaaaccccgggggcccggggggggggcccccccccaaaaaaaaatctcagggggggtttttataaaaaccccccccaaaagggtggggcccc
cttttttttttaaaaaaggggggggcccctatggtgtttgccccccacccaataaacaccccccggggggggaaaaaaaaaaaaaaaaaaggcgcgggca
aaaaaaaaaaaaaaccttttttttttgggagacaacccccctcttttttttttttatagacaaacacacccttttttttttgtgggagggaacccacccc
ccccccttgtttttttccnctccttttggagggaagggaggggagcgactacgtttttgattcgtggggagagaggggttaacatttctnatattgtgtt
gtggtggccaggcaagaaaagcagacactccaaagacaaaagtatgcgacctgatagatcggccgtggggctttctgtagtccatcatgcaancgtcgca
cacgactaactatacatagagaaatagaacattagttacnaaaactaaancttttcgcctagacatgtataaaaaaaatagatggacgagcacccccagg
aaaacggcccaccgataaacagctgtgatgagacatatgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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