XB-CLONE-4350145
Clone Name: IMAGE:7025783
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Gene:
cdk2ap1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084911 [+]
GGCTGTAAACAACAAAAAGTGTCTGAGGGAGACAGACAAGAGCCGCTCCAGTGGATCTCAGGACATTTTATTTGTTGTTGTTGCCAGATTCCTCCCCTCT
CGGCGGCGGTCATGTCTCTGGGAATGTCTTACAAGCCCAACCCGAACGCCCAGCACCCCGTCAGCAACCCCGGCTTGCAGGCTGGAAGTGCTCACTCCCC
TTCGACAAGTATGGCATCGACTGCACAGTACAGGCAGCTAGTTAATGACTACGGACCTCCATCTTTAGGATATACACAGGTTTCTAATAGTAACCAGGTA
CCTCAGAGCAAGTATGCAGAGCTTCTTGCCATAATTGAAGAGCTGGGTAAAGAAATTAGACCAACATATGCTGGCAGTAAAAGTGCTATGGAGAGACTTA
AAAGAGGTATCATCCACGCTAGGGGCTTGGTGCGGGAATGTTTGGCAGAAACAGAGCGTAATGCCAGATCCTAGCTCTGCCATGTCCCACCGTTATAAAG
CCAACAGCTATTTCTTTTTGGATTTGTGAAACCGCCAGAGTGGTCTTTCTGCAAACCAGATGGGTTCATCAGATGCATTTTGAAGAATTTTTGTTCAAAA
GCTGCTGAAGACAAAATAAAATTTTTACATATAAACAGAGTTTTCACTGAAAAGAGTTATTACTGGCTCTTTTCTCTTTTTTCAGTGAGTGCATGGATTC
TACTACATTGCTTATAAGTTACAATATATTTACATTTTATTCTTGTACATTAACACAAGCTGAGTCCTACCAATTTAAAACCATTCAATTTGTGTTCTAA
GGACATTGCATGACTTCTTTTGCCTATAGATTTGTGTTTGATATCATCTTGTAATCTACTCCATTGACTGTAATCCTTCATAGTTTTCAACAGACTATCA
TGTCATAAGACATATTTGCAGCACCTGAAAGGTCTGAGAATGTTTGTTTTTTTTCCAGTCACTGATGTCACTTTTCTGGGACAAGATTCTCTGATATATG
GAGTCTGCAAAATCTTTAAAAGTGCCCAAACAAACGGTAGACTAGTCATGTTTATTTTTGGTTAGAAATTTAGGGAAATATTAAAAAGGGAGTATATTAC
TGGCCTATGCACTTGCACGTCTGTAAACATATTTGCTAACCACTCAACATGCATGATTTATTTAAAGGAAAAATGAGAAATTTTACTTTTCTACAGGTGG
ATCTAGGTATCTGCCGTAACTGCAATTAATATACACATAAAGGCTGTGTTGGAAAAAATGAAAACTAACAATTTGCATCTTTGTATGTATTTATTACTTG
ATGTAATAAAGCTTATTTTCACTAGAAAAAAAAAAAAAAAA
5' EST: CF785711 [+]taattaatccccccccccccggctgtaaacaacaaaaagtgtctgagggagacagacaagagccgctccagtggatctcaggacattttatttgttgttg
ttgccagattcctcccctctcggcggcggtcatgtctctgggaatgtcttacaagcccaacccgaacgcccagcaccccgtcagcaaccccggcttgcag
gctggaagtgctcactccccttcgacaagtatggcatcgactgcacagtacaggcagctagttaatgactacggacctccatctttaggatatacacagg
tttctaatagtaaccaggtacctcagagcaagtatgcagagcttcttgccataattgaagagctgggtaaagaaattagaccaacatatgctggcagtaa
aagtgctatggagagacttaaaagaggtatcatccacgctaggggcttggtgcgggaatgtttggcagaaacagagcgtaatgccagatcctagctctgc
catgtcccaccgttataaagccaacagctatttctttttggatttgtgaaaccgccagagtggtctttctgcaaaccagatgggttcatcagatgcattt
ttgaagaattttgttcaanagctgctgaagacaaaataaaatttttacatataaaacagaagttttcactgaaaaaaagttattacctggctcctttttc
tctttttttcaggtgagtggcatgggatttctacctacatttggctttataaggttaccaatatattttaccatttttatttccttgggaccttaaaaca
ccaagcctgaagttccttacccaaattttaaaacccatttt
3' EST: CF785710 [+]tttttttttttttttctagtgaaaataagctttattacatcaagtaataaatacatacaaagatgcaaattgttagttttcattttttccaacacagcct
ttatgtgtatattaattgcagttacggcagatacctagatccacctgtagaaaagtaaaatttctcatttttcctttaaataaatcatgcatgttgagtg
gttagcaaatatgtttacagacgtgcaagtgcataggccagtaatatactccctttttaatatttccctaaatttctaaccaaaaataaacatgactagt
ctaccgtttgtttgggcacttttaaagattttgcagactccatatatcagagaatcttgtcccagaaaagtgacatcagtgactggaaaaaaaacaaaca
ttctcagacctttcaggtgctgcaaatatgtcttatgacatgatagtctgttgaaaactatgaaggattacagtcaatggagtagattacaagatgatat
caaacacaaatctataggcaaaagaagtcatgcaatgtccttagaacacaaattgaatggttttaaattggtaggactcagcttgtgttaatgtacaaga
ataaaatgtaaatatattgtaacttataagcaatgtagtagaatccatgcactcactgaaaaaagagaaaagagccagtaataactcttttcagtgaaaa
ctctgtttatatgtaaaattttaatttgtcttccgcagctttttgaaccaaaaattcttcaaaatgcatctgaagaaacccttctggttttggaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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