XB-CLONE-4350140
Clone Name: IMAGE:7025781
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Gene:
cdk1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077651 [+]
GGTTAGGCAAGCTGCAGCTTTAGCAGAAGGAAGCTGAGGAATCCAGCCTTGAGGAACCAGCCATGGACGAGTACACTAAAATAGAAAAGATCGGAGAGGG
CACATACGGGGTTGTGTACAAGGGTCGCCATAAAGCAACAGGCCAGGTGGTTGCAATGAAGAAAATTCGGTTGGAAAACGAAGAGGAGGGCGTCCCAAGC
ACAGCGATTAGAGAAATATCACTACTGAAAGAACTTCAGCACCCCAACATAGTTTGCCTTCTAGATGTCCTGATGCAAGACTCAAGGTTGTACCTCATCT
TTGAGTTTCTCTCCATGGATCTGAAGAAGTATTTAGACTCAATACCCAGCGGCCAGTATATAGATACAATGCTTGTTAAGAGTTACCTCTACCAGATCCT
ACAAGGGATTGTATTTTGCCACTCCAGAAGAGTGTTACACAGGGATCTGAAGCCTCAAAACCTGCTGATCGACAGCAAAGGAGTGATAAAGTTGGCAGAT
TTTGGCCTTGCCAGAGCTTTTGGCATTCCGGTTCGGGTTTACACACACGAGGTAGTGACGTTATGGTACAGAGCCCCAGAAGTGCTGTTGGGGTCAGTCC
GATATTCCACACCGGTCGACGTATGGAGCATAGGAACCATTTTTGCTGAGATTGCCACAAAGAAACCCCTCTTCCATGGCGACTCTGAAATTGACCAGCT
CTTCAGGATATTCAGAGCTCTGGGAACGCCCAACAATGAAGTGTGGCCTGAAGTGGAGTCTTTACAAGATTACAAGAACACATTCCCCAAATGGAAAGGC
GGAAATCTGTCGGCGAATGTGAAAAATATCGATAAGGATGGGCTGGACCTGCTGTCTAAAATGCTAATCTACGATCCCGCCAAGAGAATTTCTGCGCGTA
AAGCGTTGCTGCACCCCTACTTCGATGACTTGGATAAGTCCAGCCTTCCTGCCAATCAGATTAGAAATTAACACACTGAGCGTCTCTTCTCTTGGGTTTC
TGGGTGGGAATCTGATGTAAATCACTTTCGTTTTTTTTTACTGTCTGTACGTTGATGTATATGTGTGTATGTGTCTCGGCGCCCGAAGTACTTACCTTAA
AGCACCACACTCTAAATGTAAATATGGACTGACCAAACCTGTGTATAAATGCATTTCCCACACAGACATTTCATTTCCAATAAAGCTTTATTATATATTA
AAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF785714 [+]taattaatcccccccccccccggttaggcaagctgcagctttagcagaaggaagctgaggaatccagccttgaggaaccagccatggacgagtacactaa
aatagaaaagatcggagagggcacatacggggttgtgtacaagggtcgccataaagcaacaggccaggtggttgcaatgaagaaaattcggttggaaaac
gaagaggagggcgtcccaagcacagcgattagagaaatatcactactgaaagaacttcagcaccccaacatagtttgccttctagatgtcctgatgcaag
actcaaggttgtacctcatctttgagtttctctccatggatctgaagaagtatttagactcaatacccagcggccagtatatagatacaatgcttgttaa
gagttacctctaccagatcctacaagggattgtattttgccactccagaagagtgttacacagggatctgaagcctcaaaacctgctgatcgacagcaaa
ggagtgataaagttggcagattttggccttgccagagcttttggcattccggttcgggtttaacacacgaggtagtgacgttatggtacagagccccaga
agtgctgttggggtcagtccgatattccacacccgtcgacgtatggagcataggaaaccatttttgcctgagattgccacaaagaaaacccctcttccat
gggcgacctctgaaaattgacccagcttcttccggaataattcagaagcctccggggggaacgcccccaacaatggaaagttgtgggcccctggaaggtg
gaaagtctttttcccaggaattcccaggaagaccctttttccccccaaatggc
3' EST: CF785713 [+]tttttttttttttttcttttttttttttaatatataataaagctttattggaaatgaaatgtctgtgtgggaaatgcatttatacacaggtttggtcagt
ccatatttacatttagagtgtggtgctttaaggtaagtacttcgggcgccgagacacatacacacatatacatcaacgtacagacagtaaaaaaaaacga
aagtgatttacatcagattcccacccagaaacccaagagaagagacgctcagtgtgttaatttctaatctgattggcaggaaggctggacttatccaagt
catcgaagtaggggtgcagcaacgctttacgcgcagaaattctcttggcgggatcgtagattagcattttagacagcaggtccagcccatccttatcgat
atttttcacattcgccgacagatttccgcctttccatttggggaatgtgttcttgtaatcttgtaaagactccacttcaggccacacttcattgttgggc
gttcccagagctctgaatatcctgaagagctggtcaatttcaaagtcgccatgggaagaggggtttctttgtggcaatctcagcaaaaatgggtcctatg
ctccatacgtcgaccggtgtggaaaatcggaatgaccccaacagcacttctggggctccgtaccataaacgcactacctccgggtgtggtaaacccgaaa
ccgaaatgccaaagcctctgggcaaggccaaaaatctgccaactttaaacacccccttggccggcccatcaaccagggttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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