XB-CLONE-4350129
Clone Name: IMAGE:7025776
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Gene:
btf3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF785720
[+]
taattaatccccccccccccccttgcctcttggcttccatcttgctccccgcgtgtgtgcgcgcccgtcccccggctccgtaggaagcctttaaacccgc
tctgctcgccaggcgtgcccgctgtctgccttttgaagatgaaagagacaatcatgaatcaacaaaagttagccaaactgctagcacaagtgcacattga
tgcaaaggggatagtttgccgaacgtataatgttgtgcacataatagcatctgccgatgacaagaaaccgcagaactctttgaaaaaacccggagtgtat
aacacccctggaacttacgaggtgcctcccttcagcaccaaggaaccgttttttacttcatctacccccttatatacgtttctttcggttgcctaacccc
tcttcccattacgggagcatttttttaaatgtgttgccttttcaggcgatgtcttgattcccatttcccatttttttgttggcggagggaaaaagttttt
caacaaacctctgtaactaaacttcgttatgaagttccttttttttccttccccccctcgatcgttatgggcgtctttccgcctttattgcccgccccgt
ttaggggggtggagtaaaaatatttagaagtgagtcaagctttataccctttttattttttgttttttcttggtaccgcacgcctctctgcacttaacta
tgttatgtatacccggcgctactatatagtgtgttaattctgcaggaggtatatagcggggctgcactgctggctcgcagtatatttttcgtgtattaaa
atatacggcgatgtatgcggccctttcatatctgtctgaatgatcaaccccactcgctcattactatactcggtttacttctcactacttctcttccccc
cactttttttatgttcactgacaagtggttcgaatatcgttgattgacgcgatatgttgacactgtacgtggccgcttgcctcgagttgtttgggcgata
tgcgtcctctttagatgttgagtatcgtcgccttaaaacccctgagctcggcaaggcaacatggatacatgcgtgatatatttatgtgcccggggggggg
tcacgacgctcagtactccagngagcacttttttttatggcgacaagggggacccgttgttgtcggtgctgagttttatattaatttgcgtaccgttagc
ctgatctttataaaaatgaggcaggcgcaacgtaaccggttatatctttcttttatctacaccgtacggtgcgtgacggggccactcccgtgtgtatt
3' EST: CF785721
[+]tttttttttttttttgcaattcctattaagtttattctttattcatttacattttaatttccaggactgttcactttatgtggctgcaaaggcaaattat
ctgatgaatgcagttactgcagagctgctatagaaagaatttcaaatctgtggggcttttaatctcaaaggtcaaaagaaaacaacaaaaaaccacacac
aaaggcaatgcagtcacaatataaaataggagctccgtcagcttttcaagttttgttccttttagatatttttcattcagaccgattcattctttgaagc
ctcatcaaagttctcaaccagttctggtacttcatcatcttcttcctccccagaagctagtggtgctttcccatccacagattgtttaggcaaggcctct
gcaagtctccgcaagctggtaaggctgtctgctccaagctgatttaaaatgctgggcagcatctccgtaagctgcttggtctcagcatgcccagtaatgg
taaatgtgttggcagccaaggaagcctggactttggggttgttaaagtgaataactgttccttggtttgtaaacatattcacctcctctatgccagagat
gttattcactccaagttttttcagagagaaatgcagtttcttatcatcggcagttgctgttctgtgcacaaccttcttctttctgcgggctgtccccttt
cacccatgcgcacttgtgcttgcagtttggctaacttttcttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: