XB-CLONE-4350126
Clone Name: IMAGE:7025775
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Gene:
tspan14
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080135 [+]
CGGCTGTGTTCTGCTTGTTTGGGTTGGATGCTGTAATTTTCAACAGGGAGAGTTTGCTTAGTCCGGGTATTTTTGCTGTAGCCTTTTTTTTCCCTCCGTG
CCATGGAATCTTGAAAAAGTGTTGTGATAGTGGGGCAGTGCGGTAATCTTAACTGCTGCAAACATGCACTACTACCGGTACAGCACTGCAGAAGTCAGCT
GTTGTTATAAGTACCTCCTGTTTAGCTACAATATCATATTCTGGCTATCGGGAATGGTTCTTTTAGGAGTTGGTTTGTGGGCATGGAGTGAAAAAGGCAT
ATTGTCAGATATTACAAAAGTAACACGTCTCCATGGATTTGACCCAGTGTGGCTAGTCCTGGTGGTTGGTGTCATCATGTTTACTCTTGGCTTTGCTGGC
TGTGTTGGTGCTTTACGAGAAAATATCTGCCTTCTGAAATTTTTTTGTGGTGCTATAGTCTTGATTTTCTTCATGGAGCTCGCTGTTGCAGTTCTTGCCT
TCCTATTCCAGGACTGGGTGAAAGACCGAGCCAAGGATTTTTTTGAGAACAATATAAAATCATACAGAGATGATATTGACTTGCAGAACCTCATAGATTC
CCTTCAAAAAGCGAACCAGTGTTGTGGTGCTGTGTCCCCAGATGACTGGGACCTAAATGAATATTTCAACTGCAGTGTTGAAAATCCAAGTCGTGAGAGG
TGTGGTGTTCCTTTTTCTTGTTGTGTTCCTGATCCAGCTCAAACAGTTGTGAACACACAGTGTGGTTATGATGCTCGCAGAAAGAAGCCCAACGAGAGAC
CAAATACAACATTCTCAAAAGGCTGTATATCTGCACTGGAGGCATGGTTACCGAGAAACATATATATTGTGGCTGGAGTTTTTCTAGTGATCTCAATTTT
ACAGATTTTTGGTATTTACCTTGCTCGGACACTGATGTCAGATATTGAAGCAGTGAAGGCCGGTTACCGCTTTTAAGGGCGACCCTCATCGTTCAAAGTC
ATTACAGAGACTTTCTGTATTTTTTCCCATCTTTTAAATCTTTGTTTGCAGTTAGAGACTTTTCAACATTGCCATTATCAATAAAGATCTGCACAGAAGG
ACCCGTTGTAATTAATCTAGGGTTTCCGTGCATCCTGCAATATGTAAAAGGATAGACTTGAAATGTTACTATATTTAACCACTAGTCATTTATTATTCCT
CCTGGAACTTCTCCAAGAAGAGACTACACTTTGTTTTGAAGAAAGGTTCTCTGAATCTCCCTACTATTCTGGCTTTACATTTTCCTTCAGCTTTTTTCTT
CAGTGCTGGAATATATGTCGACCCGGATTACTTATTGATGGACAGTAAACATTCATATGAGTTCTCTGGACAATGAACTCAGGCATTTACCTTCATCTAA
CAATGTCATTAAACCTTTTTTAAACCGAATGATTTACAAGTTCACCAAAGCCTTACCAAGTTAAGACACGTTCACTTATGTCAATAATGCTGCCATAGAT
TTTTAGAGGTATAACATAACTTTTGTTTGCCAACATGTCAAGGAGTAATGTTTTACTATAAAAAAAATGTATTACTACTGTAAATCATTACATTCACAAG
TATCTTATTTATGGAAAGGAAAACCAAGTAATCCCCAAAAATACTCAATTTGAAATCTAAACATAAGAGATATCAAGGGCTATTAGTCTGGGATTTGACT
GTAGTTCTTTGTATTTCACTGTTGGAGAAATATAGGTTATCCCAAAGGAATTATTTCTACTGTGAAAAGCAAATACATGATTCTCTGTTGATATAAGGAC
ACAACAGTGTCAACATCATTGAGTTGTTTTTTGTTTTGTTTTTTTATATAAAAAAAACCCACTATATTATTGCAGGATATATGTTCTTTTACCAGAGAAT
CTGTTGTAGAGTTACGTGTTTATTTTGTAAAAAAAATTATATGGGAAAAAATTGAATATAGCTGTATCTATTGCTTTACAGATGATCACAGAAGTCTATA
GATTTTACTTAGTAGCACAATTTGTTTTAAATATGTACAATATTATATACCTGCATATCCACACATACATTAAGGGCTGTGTGCCCATTTGGAGTTACTT
CTTTCAAATGGTTCCCCAGTAATGTTAAGAGTAGTTATTGTTTCATTTCAGCGCTTACTCTGTGTGGCTTTAGCAGCACATTCATGATTTGATAACAGTT
TGCATTCAGGCTTAGGCTGGAAATGTATTGTCCATACACCAGCATGATCAAATTCAGCATGGCATTTCTAAATACACATTTATTGTATAACTAGGAAGGG
GCTTGCTCCTGTGTCAAGTTCTAAATGAGCTGGTCCTGACTTAGAGCCCCGTTTGAAGATATTGTTTGTAGTAAACAAACTATGGGGCCCACTTCAGCCA
TCTGACTGAAATTTGAAATTAACCACGTATTGGGGTATATTTATAAAAGCCTGCCAAACATTTTGCAGCAAAAACAGTGTACATTTGGTGGAACATAGAG
AGCATGTTTATACAGCTCCTGTATATTTTATTTACGCCATACTAACCCTGAAAATAAAATCATTGTTTTACAACTCTAGACTGTAGACCTATTTAAGTGG
TGGTTCACCTTTAAGTTAACTTCTAATATGTTATATTATGGCTAATTTTTGGCAACTTTTTGAATTGGTCTACCTTTTTTATTTGTATAGTTTTTTTAAT
TAATCGTGACCCCCCAGCAGCCAAAAAAAAAAATTTTATTGTTATTTTTTATACTTTATCTTTGTATTCAGACCCTCTTCTATTTATATTGCAGTCTCTC
AGACTCAAACTACTGCCTGTTTGCTAGGGTAAGTTAGACCCTAGCAACCAGTAGGTACTGAAATCCCAAACCAGAGAGCTGAACAAGAAGCTAAAGAATA
AGAATAAAAAATAAAGACCAATTGCACATTGCATTAGAATAGCACTATCTACATCAGGGGTCCCCAATCTTTCTTACTCGTAAACCACAGCCAAATGTAA
AAAGACTTAGGGGAGCAACAACAAGTATCCTAAAAGTTCATGGAAGTGGCAAATAAGAACTAAGGTTGCCTATTAGGTAGCCTCTATGAACACACTCAAC
TTACAAGAGGCTTTATTTGGTAGTAAATCTTTTTTCGTTTTTATTCAACCAAAGCTTGCCACTGAGGCACAAATTAAAATAATAACTACCTGGTTTGGGG
GCACTGAGAGCAACAACCAAGGGGTTGGTGAGCAGCACGTTACACACTTCTAAATATACCCTATTATGTATTCCCTAGGTGTTTACTATTTATGGTTGAT
TTTTAAGTTACCCAGTTATTCAATAACAGACATTTTCAGCAAAAAAAAAAAAAAAA
5' EST: CF785723 [+]taattaatccccccccccccccggctgtgttctgcttgtttgggttggatgctgtaattttcaacagggagagtttgcttagtccgggtatttttgctgt
agccttttttttccctccgtgccatggaatcttgaaaaagtgttgtgatagtggggcagtgcggtaatcttaactgctgcaaacatgcactactaccggt
acagcactgcagaagtcagctgttgttataagtacctcctgtttagctacaatatcatattctggctatcgggaatggttcttttaggagttggtttgtg
ggcatggagtgaaaaaggcatattgtcagatattacaaaagtaacacgtctccatggatttgacccagtgtggctagtcctggtggttggtgtcatcatg
tttactcttggctttgctggctgtgttggtgctttacgagaaaatatctgccttctgaaatttttttgtggtgctatagtcttgattttcttcatggagc
tcgctgttgcagttcttgccttcctattccaggactgggtgaaagaccgagccaaggatttttttgagaacaatataaaatcatacagagatgatattga
cttgcagaacctcatagattccccttcaaaagcgaaccagtgttgtggtgctgtgtccccagatgactgggacctaaatgaatatttcaactgcagtgtt
tgaaaatccaagtcgtgagaaggggtgggggttccctttttccgggtggggtcccggataccagcttcaaccagttggtgaacaccacagtggggggtta
tggatgcctccccaaaaaaaaaaaa
3' EST: CF785722 [+]tttttttttttttttgctgaaaatgtctgttattgaataactgggtaacttaaaaatcaaccataaatagtaaacacctagggaatacataatagggtat
atttagaagtgtgtaacgtgctgctcaccaaccccttggttgttgctctcagtgcccccaaaccaggtagttattattttaatttgtgcctcagtggcaa
gctttggttgaataaaaacgaaaaaagatttactaccaaataaagcctcttgtaagttgagtgtgttcatagaggctacctaataggcaaccttagttct
tatttgccacttccatgaacttttaggatacttgttgttgctcccctaagtctttttacatttggctgtggtttacgagtaagaaagattggggacccct
gatgtagatagtgctattctaatgcaatgtgcaattggtctttattttttattcttattctttagcttcttgttcagctctctggtttgggatttcagta
cctactggttgctagggtctaacttaccctagcaaacaggcagtagtttgagtctgagagactgcaatataaatagaagagggtctgaatacaaagataa
agtataaaaaataacaataaaatttttttttttggctgctggggggtcacgatttatttaaaaaaactatacaaataaaaaaggtagaaaaattcaaaaa
agttgccaaaaattagcccctaatattaacctatttagaatttactttaaaagtgaaaccaccacttaaaataagtctacaggtctagaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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